Mouse IL-23 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # M2300
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), Heparin Plasma (50 µL)
Sensitivity
4.17 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Serum, Heparin Plasma)
Product Summary for Mouse IL-23 Quantikine ELISA Kit
The Quantikine Mouse IL-23 immunoassay is a 4.5 hour solid-phase ELISA designed to measure IL-23 in cell culture supernates, mouse serum, and plasma. It contains Sf 21-expressed recombinant mouse IL-23 and antibodies raised against the recombinant p19 and p40 subunits. This immunoassay has been shown to accurately quantitate the recombinant mouse IL-23. Results obtained using natural mouse IL-23 showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-23.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse IL-23. This assay does not recognize free p19 or p40 subunits.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 49.3 | 107 | 344 | 50.4 | 116 | 333 |
| Standard Deviation | 3.72 | 5.84 | 22.7 | 3.69 | 7.14 | 18.6 |
| CV% | 7.5 | 5.5 | 6.6 | 7.3 | 6.2 | 5.6 |
Recovery for Mouse IL-23 Quantikine ELISA Kit
The recovery of mouse IL-23 spiked to three levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 103 | 99-108 |
| Heparin Plasma (n=4) | 99 | 88-117 |
| Serum (n=4) | 95 | 90-101 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of mouse IL-23 in each matrix were diluted with Calibrator Diluent and assayed.
Scientific Data Images for Mouse IL-23 Quantikine ELISA Kit
Mouse IL-23 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-23
IL-23 (Interleukin-23) is a disulfide-linked cytokine composed of a p19 subunit that is unique to IL-23 and a p40 subunit that is shared with IL-12. It is produced by activated macrophages, microglia, and monocyte-derived dendritic cells in response to pathogens. IL-23 induces the earliest recruitment of neutrophils to the site of infection and promotes the development and maintenance of Th17 cells. It also enhances the development of Th17 mediated autoimmunity and tumor progression. IL-23 signals through a receptor complex consisting of IL-12 R beta 1 and IL-23 R. This complex is expressed in mouse Th1 and Th2 cells, bone marrow dendritic cells, activated macrophages and CD4+ CD45Rb(low) memory T cells.
Long Name
Interleukin 23
Alternate Names
IL-23 p19/IL-12 p40, IL-23p19/IL-12p40, IL23, SGRF
Gene Symbol
IL23A
Additional IL-23 Products
Product Documents for Mouse IL-23 Quantikine ELISA Kit
Product Specific Notices for Mouse IL-23 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.