Mouse IL-12 p40 NonAllele-specific Quantikine ELISA
R&D Systems, part of Bio-Techne | Catalog # MP400
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)
Sensitivity
2.7 pg/mL
Assay Range
11.7-750 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse IL-12 p40 NonAllele-specific Quantikine ELISA
The Quantikine Mouse IL-12/IL-23 p40 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse p40 subunit in IL-12 p70, IL-23, p40 homodimer, and p40 monomer in cell culture supernates, mouse serum, and plasma. It contains recombinant mouse IL-12/23 p40 homodimer and antibodies raised against the p40 subunit of recombinant mouse IL-12 p70. This immunoassay has been shown to accurately quantitate the recombinant mouse IL-12/IL-23 p40. Results obtained using natural mouse IL-12/IL-23 p40 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-12/IL-23 p40.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse IL-12/IL-23 p40
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 26 | 21 | 27 |
| Mean (pg/mL) | 33.3 | 56.4 | 397 | 36.5 | 62.7 | 405 |
| Standard Deviation | 2.3 | 3.0 | 17.2 | 3.8 | 6.5 | 33.7 |
| CV% | 6.9 | 5.3 | 4.3 | 10.4 | 10.4 | 8.3 |
Recovery for Mouse IL-12 p40 NonAllele-specific Quantikine ELISA
The recovery of mouse IL-12/IL-23 p40 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 104 | 98-108 |
| EDTA Plasma (n=4) | 99 | 81-112 |
| Heparin Plasma (n=4) | 95 | 80-109 |
| Serum (n=4) | 105 | 86-117 |
Linearity
To assess the linearity of the assay, four or more samples containing and/or spiked with various concentrations of mouse IL-12/IL-23 p40 in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse IL-12 p40 NonAllele-specific Quantikine ELISA
Mouse IL-12/IL-23 p40 Non Allele-specific ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-12/IL-23 p40
IL-12 (Interleukin-12) is a secreted heterodimeric cytokine that contains disulfide-linked p35 and p40 subunits. The p40 subunit is also a subunit of the heterodimeric IL-23. IL-12 is produced by macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, keratinocytes, microglia, and non-germinal center B cells. It acts through a receptor complex that contains the ligand binding IL-12 R alpha 1 and the signal transducing IL-12 R alpha 2. IL-12 can enhance cytotoxic activity and induce Interferon-gamma production in NK cells, T cells and dendritic epidermal T cells. In conjunction with IL-23 and IL-27, IL-12 promotes the development of a Th1 immune response.
Long Name
Interleukin 12/Interleukin 23 p40
Alternate Names
CLMF2, IL-12B, IL12 p40, IL12B, NKSF2
Entrez Gene IDs
Gene Symbol
IL12B
Additional IL-12/IL-23 p40 Products
Product Documents for Mouse IL-12 p40 NonAllele-specific Quantikine ELISA
Product Specific Notices for Mouse IL-12 p40 NonAllele-specific Quantikine ELISA
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.