Mouse/Rat FGF-21 Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # MF2100
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)
Sensitivity
13.4 pg/mL
Assay Range
31.3-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse/Rat FGF-21 Quantikine ELISA Kit
The Quantikine Mouse/Rat FGF-21 immunoassay is a 4.5 hour solid-phase ELISA designed to measure FGF-21 in mouse or rat cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse FGF-21 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse FGF-21. Results obtained using natural mouse or rat FGF-21 showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural FGF-21.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse, Rat
Specificity
Natural and recombinant mouse and rat FGF-21
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma - Mouse and rat samples were evaluated for the presence of FGF-21 in this
assay.
ND=Non-detectable
| Mouse Samples | Mean (pg/mL) | Range (pg/mL) | Standard Deviation (pg/mL) |
| Serum (n=20) | 887 | 115-3212 | 874 |
| EDTA plasma (n=20) | 878 | 219-1650 | 385 |
| Heparin plasma (n=20) | 877 | 283-2280 | 622 |
| Rat Samples | Mean of Detectable (pg/mL) | % Detectable | Range (pg/mL) |
| Serum (n=20) | 402 | 90 | ND-2222 |
| EDTA plasma (n=20) | 741 | 100 | 88-1876 |
| Heparin plasma (n=20) | 970 | 95 | ND-3428 |
Cell Culture Supernates - L-929 mouse fibroblast cells (0.5 x 105
cells/mL) were cultured in
MEM (NEAA) supplemented with 10% equine serum, 2 mM L-glutamine, 100 U/mL penicillin,
and 100 μg/mL streptomycin sulfate. The cells were either unstimulated or stimulated for
3 days with 1 μg/mL LPS or 10 μg/mL PHA and 10 ng/mL PMA. Aliquots of the cell culture
supernates were assayed for levels of mouse/rat FGF-21.
| Condition | Observed Levels (pg/mL) |
| Unstimulated | 387 |
| LPS | 300 |
| PHA and PMA | 185 |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 84.4 | 226 | 754 | 94.5 | 231 | 745 |
| Standard Deviation | 5.24 | 9.60 | 19.7 | 6.61 | 10.8 | 31.0 |
| CV% | 6.2 | 4.2 | 2.6 | 7.0 | 4.7 | 4.2 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 118 | 317 | 1032 | 120 | 302 | 977 |
| Standard Deviation | 7.35 | 13.5 | 25.2 | 8.91 | 18.0 | 65.9 |
| CV% | 6.2 | 4.3 | 2.4 | 7.4 | 6.0 | 6.7 |
Recovery for Mouse/Rat FGF-21 Quantikine ELISA Kit
The recovery of FGF-21 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Mouse Cell Culture Samples (n=6) | 96 | 88-101 |
| Mouse EDTA Plasma (n=4) | 97 | 86-104 |
| Mouse Heparin Plasma (n=4) | 93 | 83-101 |
| Mouse Serum (n=4) | 95 | 83-103 |
| Rat Cell Culture Samples (n=2) | 91 | 87-95 |
| Rat EDTA Plasma (n=4) | 101 | 93-119 |
| Rat Heparin Plasma (n=4) | 97 | 94-104 |
| Rat Serum (n=4) | 100 | 93-108 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of FGF-21 in each matrix were diluted with the appropriate Calibrator Diluent and assayed.
Scientific Data Images for Mouse/Rat FGF-21 Quantikine ELISA Kit
Mouse/Rat FGF-21 ELISA Cell Culture Supernate Standard Curve
Mouse/Rat FGF-21 ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: FGF-21
FGF-21 (Fibroblast Growth Factor 21) is a member of an FGF subfamily that also includes FGF-19 and FGF-23. FGF-19 subfamily members lack a heparin binding domain, enabling them to freely circulate as endocrine factors and diffuse within tissues. FGF-21 signals through Klotho beta in complex with FGF receptors. It regulates cellular metabolism including glucose uptake in adipocytes and cellular sensitivity to insulin. It is basally expressed in the pancreas, thymus, liver, and adipose tissue and is upregulated in liver, muscle, and fat in obesity and diabetes. FGF-21 also plays a role in cell survival and proliferation, mesenchymal stem cell differentiation, circadian rhythm, and controlling reproductive capacity.
Long Name
Fibroblast Growth Factor 21
Alternate Names
FGF21
Gene Symbol
FGF21
Additional FGF-21 Products
Product Documents for Mouse/Rat FGF-21 Quantikine ELISA Kit
Product Specific Notices for Mouse/Rat FGF-21 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.