Mouse E-Selectin/CD62E Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MES00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL)
Sensitivity
4.7 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Product Summary for Mouse E-Selectin/CD62E Quantikine ELISA Kit
The Quantikine Mouse soluble E-Selectin (sE-Selectin) Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse sE-selectin levels in cell culture supernates, serum, and EDTA plasma. It contains NS0-expressed recombinant mouse sE-selectin and antibodies raised against the recombinant protein. Results obtained for naturally occurring mouse sE-selectin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse sE-selectin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse E-Selectin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 52 | 152 | 464 | 49 | 144 | 440 |
| Standard Deviation | 4.6 | 9.5 | 41 | 4.3 | 10 | 29 |
| CV% | 8.8 | 6.3 | 8.8 | 8.8 | 6.9 | 6.6 |
Recovery for Mouse E-Selectin/CD62E Quantikine ELISA Kit
The recovery of mouse sE-Selectin spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=6) | 95 | 83-102 |
| EDTA Plasma (n=4) | 101 | 78-120 |
| Serum (n=10) | 98 | 83-118 |
Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with high concentrations of mouse sE-Selectin in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse E-Selectin/CD62E Quantikine ELISA Kit
Mouse E-Selectin/CD62E ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: E-Selectin/CD62E
E-Selectin, also known as CD62E, is expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha. It is a lectin that binds to sialylated or fucosylated molecules on neutrophils, monocytes, and a subset of memory T cells. E-Selectin mediates the initial attachment of circulating leukocytes to the blood vessel wall during inflammation. This interaction permits leukocytes to roll along the vascular endothelium. Subsequent ICAM-1 and VCAM-1 mediated adhesion leads to extravasation of the leukocyte through the blood vessel wall into the extracellular matrix tissue.
Alternate Names
CD62E, ELAM1, LECAM2, SELE
Gene Symbol
SELE
Additional E-Selectin/CD62E Products
Product Documents for Mouse E-Selectin/CD62E Quantikine ELISA Kit
Product Specific Notices for Mouse E-Selectin/CD62E Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.