Mouse Dkk-1 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MKK100
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
29.6 pg/mL
Assay Range
62.5-4000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse Dkk-1 Quantikine ELISA Kit
The Quantikine Mouse Dkk-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure Dkk-1 in cell culture supernates, mouse serum, and plasma. It contains NS0-expressed recombinant mouse Dkk-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse Dkk-1. Results obtained using natural mouse Dkk-1 showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Dkk-1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse Dkk-1. This assay cross-reacts 94% with recombinant Rat Dkk-1 and 26% with recombinant Human Dkk-1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity observed with 1 or more species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 230 | 478 | 1487 | 219 | 481 | 1492 |
| Standard Deviation | 20.1 | 29.7 | 80.8 | 20.8 | 44.7 | 144 |
| CV% | 8.7 | 6.2 | 5.4 | 9.5 | 9.3 | 9.7 |
Recovery for Mouse Dkk-1 Quantikine ELISA Kit
The recovery of mouse Dkk-1 spiked to three levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 99 | 88-107 |
| EDTA Plasma (n=4) | 109 | 93-117 |
| Heparin Plasma (n=4) | 113 | 104-119 |
| Serum (n=4) | 113 | 100-120 |
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of mouse Dkk-1 in each matrix were diluted with Calibrator Diluent and assayed.
Scientific Data Images for Mouse Dkk-1 Quantikine ELISA Kit
Mouse Dkk-1 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Dkk-1
Dkk-1 (Dickkopf related protein 1) is a secreted antagonist of Wnt/beta-Catenin signaling. It interacts with LRP-6, and internalized complexes of Dkk-1/LRP-6/Kremen 2 can downregulate Wnt signaling. The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis. In addition, Dkk-1 can act as either a tumor suppressor or promoter of metastasis.
Long Name
Dickkopf-1
Alternate Names
Dkk1
Gene Symbol
DKK1
Additional Dkk-1 Products
Product Documents for Mouse Dkk-1 Quantikine ELISA Kit
Product Specific Notices for Mouse Dkk-1 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.