Mouse CCL22/MDC Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MCC220
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (17 µL), EDTA Plasma (17 µL), Heparin Plasma (17 µL)
Sensitivity
1.8 pg/mL
Assay Range
7.8-500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse CCL22/MDC Quantikine ELISA Kit
The Quantikine Mouse MDC Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse MDC in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse MDC and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse MDC showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse MDC.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse MDC
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 24 | 24 | 24 |
| Mean (pg/mL) | 19.9 | 51.8 | 382 | 20.0 | 52.6 | 383 |
| Standard Deviation | 1.3 | 2.5 | 19.2 | 1.7 | 2.9 | 28.7 |
| CV% | 6.5 | 4.8 | 5.0 | 8.5 | 5.5 | 7.5 |
Recovery for Mouse CCL22/MDC Quantikine ELISA Kit
The recovery of mouse MDC spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=7) | 102 | 87-112 |
| EDTA Plasma (n=4) | 94 | 89-98 |
| Heparin Plasma (n=4) | 90 | 80-109 |
| Serum (n=5) | 91 | 82-100 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse MDC in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse CCL22/MDC Quantikine ELISA Kit
Mouse CCL22/MDC ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL22/MDC
Macrophage-derived chemokine (MDC), also named stimulated T cell chemotactic protein (STCP-1) and ABCD-1, and now designated as CCL22, is a CC chemokine initially isolated from clones of monocyte-derived macrophages. At the amino acid sequence level, MDC shows less than 35% identity to other CC chemokine family members. Human MDC is expressed in dendritic cells, macrophages and activated monocytes. In addition, MDC expression is detected in thymus, lymph node and appendix tissues. At the amino acid sequence level, mouse and human MDC share 64% identity and 83% similarity.
Additional CCL22/MDC Products
Product Documents for Mouse CCL22/MDC Quantikine ELISA Kit
Product Specific Notices for Mouse CCL22/MDC Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.