Mouse Chitinase 3-like 1/YKL-40 DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY2649
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
31.2-2000 pg/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Mouse
Mouse Chitinase 3-like 1/YKL-40 DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Mouse Chitinase 3-like 1/YKL-40 DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse Chitinase 3-like1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
100 µL
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Mouse Chitinase 3-like 1/YKL-40 DuoSet ELISA
Mouse Chitinase 3-like 1 ELISA Standard Curve
Kit Contents for Mouse Chitinase 3-like 1/YKL-40 DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990)
Plate Sealers: ELISA Plate Sealers (Catalog # DY992)
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Chitinase 3-like 1/YKL-40
Chitinase 3-like 1/YKL-40 (CHI3L1), also called BRP39 in mouse, is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family although it does not show chitotriosidase activity. CHI3L1 is expressed by articular chondrocytes, synovial cells, activated monocyte-derived macrophages, neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells. CHI3L1 binds to chitin and heparins, and it enhances cell adhesion, proliferation and tumor angiogenesis. Circulating CHI3L1 is elevated during inflammation and connective tissue remodeling such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis. It is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary.
Alternate Names
CHI3L1, Chitinase 3 like 1, HCgp39, YKL-40
Gene Symbol
CHI3L1
Additional Chitinase 3-like 1/YKL-40 Products
- All Products for Chitinase 3-like 1/YKL-40
- Chitinase 3-like 1/YKL-40 cDNA Clones
- Chitinase 3-like 1/YKL-40 ELISA Kits
- Chitinase 3-like 1/YKL-40 Luminex Assays
- Chitinase 3-like 1/YKL-40 Lysates
- Chitinase 3-like 1/YKL-40 Primary Antibodies
- Chitinase 3-like 1/YKL-40 Proteins and Enzymes
- Chitinase 3-like 1/YKL-40 Simple Plex
Product Documents for Mouse Chitinase 3-like 1/YKL-40 DuoSet ELISA
Product Specific Notices for Mouse Chitinase 3-like 1/YKL-40 DuoSet ELISA
For research use only
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