Human Pax2 Antibody

Detection of Rat Human Pax2 Antibody by Immunohistochemistry

Ablation of AAV-PdynP+ spinal dorsal horn (SDH) neurons does not affect behavioral responses evoked by the optical stimulation of A beta fibers, nociceptive mechanical force, and heat in rats. (A) Enhanced green fluorescent protein (EGFP) expression (green) in the L4 SDH at 4 weeks after intra-SDH microinjection of AAV-PdynP-DTR-EGFP (indicated by an upper schematic illustration). Colocalization of EGFP and PAX2 (red) in the SDH (indicated by arrowheads). Scale bar, 100 μm. The percentage of co-expressing neurons was quantified (n = 3 rats tested, 94 total EGFP+ cells quantified, and 339 total PAX2+ cells quantified). (B) Diphtheria toxin receptors (DTR) immunofluorescence in the SDH after administration of diphtheria toxin (DTX: 50 μg/kg, i.p., two injection 72 h apart) or PBS. Scale bar, 200 μm. (C) Immunohistochemical analysis and quantification of PAX2+ neurons in the superficial laminae of the ipsilateral and contralateral SDH at 4 weeks after DTX injection (n = 6 rats tested, PAX2+ neurons: 992 [ipsi] and 1574 [contra] cells quantified). Scale bar, 50 μm. ****P < 0.0001, Unpaired t-test. (D) Paw withdrawal behaviors by light (score), von Frey filaments (threshold), and heat (latency) at 4 weeks after DTX or PBS injection (n = 7–9 rats). Data shown as mean ± SEM. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35813063), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Rat Human Pax2 Antibody by Immunohistochemistry

AAV-PdynP injected into rat spinal dorsal horn (SDH) captures a subset of inhibitory neurons. (A) tdTomato (tdT) expression in the fourth lumbar (L4) SDH at 4 weeks after microinjection of AAV-PdynP-tdT. Schematic illustration indicates intra-SDH microinjection of the AAV vector. Scale bar, 200 μm. (B) Immunolabeling of tdT+ cells (red) with lamina-selective markers (green, NK1R; blue, IB4) in the L4 SDH. Scale bar, 200 μm. Quantification of the distribution of tdT+ cells (n = 3 rats tested, 272 total tdT+ cells quantified). (C) Immunofluorescence of prodynorphin (PDYN; green) in tdT+ SDH neurons (red). Scale bar, 200 μm (above), 20 μm (below). Arrowheads indicate tdT+ PDYN+ neurons. The percentage of tdT+ PDYN+ neurons per total tdT+ neurons was quantified (n = 4 rats tested, 1,281 total tdT+ cells quantified). (D) No colocalization of tdT (red) with NK1R (green). Scale bar, 50 μm. The percentage of co-expressing neurons was quantified (n = 3 rats tested, 187 total tdT+ cells quantified). (E) Firing patterns of tdT+ SDH neurons, and the percentage of neurons displaying each pattern. APs were evoked by current injection (80 pA). (F) Colocalization of tdT (red) with PAX2 (blue) and VGLUT2 (green) in the superficial laminae. Arrowheads and arrows indicate tdT+PAX2+ and tdT+VGLUT2+ neurons, respectively. Scale bar, 50 μm. The percentage of co-expressing neurons was quantified (n = 4 rats tested, 456 total tdT+ cells quantified). Data shown as mean ± SEM. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35813063), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Pax2 by Immunohistochemistry

MiR34/449 tunes optimal Satb2 expression in the Ctip2 IN subpopulation.(A) Immunostaining for Satb2, Ctip2,&Pax2 in dorsal&intermediate spinal cord at P14. Note that Satb2on INs display two molecular identities, i.e., the Ctip2on or Pax2on subpopulations. Comparison with WT/Ctrl reveals an increase in the cell number of Satb2on;Ctip2on INs in the intermediate spinal cord of cervical/brachial sections upon MiR34/449 deletion. Boxed areas indicate higher magnification regions from the intermediate&dorsal regions of each image. Arrows highlight the presence of dual-labeled Satb2on;Ctip2on cells (yellow) or dual-labeled Satb2on;Pax2on cells (magenta) among the Satb2on population. (B–C) Quantifications of cell number from (A). (D) Quantifications of the Satb2on;Ctip2on&Satb2on;Pax2on subpopulations normalized against total Satb2on cell numbers&are shown as a %age. (E) Comparison of the spatial distribution of individual Satb2on;Ctip2on INs in the cervical/brachial spinal cord of indicated mice (Ctrl, grey; Mir34bc/449 DKO&Mir34/449 TKO, magenta). Frequency distributions along the medio-lateral (top)&dorso-ventral (right) axes represent non-linear regressions. (F) A contour density plot of Satb2on;Ctip2on INs indicates that Satb2on;Ctip2on INs are mainly located in the medial spinal cord of both MiR34/449 mutant&Ctrl mice. Contour lines represent density at the 30th–90th %iles. Data analyzed from more than 25 sections of at least five independent spinal cords in (E)&(F). (G) Schematic illustrating the increased number of Satb2on;Ctip2on INs in the MiR34/449 mutant spinal cord. Data are presented as mean ± SD. One-way ANOVA with Tukey’s multiple comparisons test. * denotes p<0.05; ‘NS’ denotes ‘not significant’. Scale bars represent 100 μm. Image collected&cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33787491), licensed under a CC-BY license. Not internally tested by R&D Systems.