Human Neudesin Antibody

Detection of Human Neudesin by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line, IMR-32 human neuroblastoma cell line, and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Neudesin Monoclonal Antibody (Catalog # MAB6714) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Neudesin at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Neudesin in Human Brain.

Neudesin was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human Neudesin Monoclonal Antibody (Catalog # MAB6714) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Neudesin by Immunohistochemistry

Characterization of NENF expression and release in colorectal cancer patients and DLD-1 and HT-29 cell lines. NENF expression at gene (a) and protein (b,c) levels in NM (n = 10) and colorectal cancer (CRC) tissues (n = 20). Original magnification, 20×; scale bar, 20 µm. The columns represent the mean ± SEM relative to PPIA. The Mann–Whitney test was used to compare NM vs. CRC results. The differences are statistically non-significant. Serum NENF concentration in colorectal cancer patients (n = 41) compared to the control group (n = 15) (d). The Mann–Whitney test was used to compare serum NENF concentration in C vs. CRC results. Statistical significance: * p ≤ 0.05. NENF expression after treatment with 1 µM of P4 in DLD-1 cell line (e) and HT-29 cell line (f) (n = 3 independent experiments). The Mann–Whitney test was used to compare NENF expression in C vs. CRC results in DLD-1 and HT-29 cells. The differences are statistically non-significant. NENF concentration in the medium after treatment with 1 µM of P4 in DLD-1 cell line (g) (n = 6) and HT-29 cell line (h) (n = 6). The Mann–Whitney test was used to compare NENF concentration in the medium C vs. P4 results of DLD-1 cells, statistical significance: ** p ≤ 0.01. Double staining for PGRMC1 and NENF in NM tissues (n = 10) and colorectal cancer (n = 20) (i). PGRMC1-positive cells are in red, NENF-positive cells are in green, nucleus localization in cells is in blue, and PGRMC1 and NENF merged staining is in orange, scale bar, 20 μm. Abbreviations: C, control/non-treated group; CRC, colorectal cancer; NENF, neudesin; NM, normal mucosa; P4, progesterone; PGRMC1, progesterone receptor membrane component 1; PPIA, peptidylprolyl isomerase A; SD, standard deviation; SEM, standard error of the mean; and vs., versus. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37894441), licensed under a CC-BY license. Not internally tested by R&D Systems.