IFN-kappa was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human IFN-kappa Monoclonal Antibody (Catalog # MAB102471) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in keratinocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human IFN-kappa Antibody

Application

Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-kappa

Interferon kappa, also known as IFN-kappa, is a 207 aminoacids protein that in humans is encoded by the IFNK gene. IFN-kappa is a member of the type I interferon family which also includes IFN-alpha, -beta, -epsilon, and -omega. Type I interferons are a group of related glycoproteins that play an important role in host defenses against viral infections. Human IFN-kappa has been detected in keratinocytes, monocytes, and monocyte-derived dendritic cells and is reported to have contact-dependent antiviral activity. Human papillomavirus (HPV) 16 oncogene expression, which is necessary for the development of cervical cancer, has been shown to down-regulate human IFN-kappa expression.

Long Name

Interferon kappa

Alternate Names

IFNK, IFNkappa

Entrez Gene IDs

56832 (Human); 387510 (Mouse)

Gene Symbol

IFNK

UniProt

Q90W0

Additional IFN-kappa Products

Product Documents for Human IFN-kappa Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human IFN-kappa Antibody

For research use only

Citations
Reviews

Human IFN-kappa Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB102471

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all IFN-kappa Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human IFN-kappa Antibody

IFN-kappa in Human Skin.