Simple Plex™ Assays for Cell Therapy Research and Development
Automated Simple Plex assays on the Ella™ platform offer high-quality immunoassay data and unparalleled efficiency for your cell therapy workflow. See how Simple Plex assays are used in the cell therapy workflow. Next, meet Ella and request pricing. Finally, choose the Simple Plex Assay that best meets your needs.
Table of Contents
Simple Plex Assays on Ella Expedite Cell Therapy Process Development
- Ella reduces the manual operation of an immunoassay by 80 percent. Simply dilute samples and add them to the cartridge.
- With a total time to result of just 90 minutes, Ella gives you results in a fraction of the time of a manual assay.
- Sample volume requirements of 25 µL or less and triplicate measurements from every sample well reduce consumption of your precious samples.
- Measure viral titers and characterize transduced cells with Simple Plex assays designed to meet your needs.
Simple Plex Assays are Consistent And Reliable
Simple Plex assays on Ella deliver the same data regardless of instrument, operator or location.
- Volume independent assays that are robust to how much sample is added
- Factory calibrated cartridges minimize inter-lot variability allowing you to get the same measurement anywhere
- Reduction in manual steps eliminates operator error
Figure (Right): Testing across 3 sites, 11 users and 9 Ella instruments. Four different assays were processed for CCL2, IL-6, TNF α and VEGF-A. Eight unique serum samples with 2 controls for a total of 704 answers.
Simple Plex Solutions For Cell Therapy Manufacturing
The manufacturing of gene-modified immune cells is a multistep process that requires rigorous product characterization at various points throughout the workflow. Ella gives you the critical information you need faster, with superior sensitivity and reproducibility that supports regulatory compliance. Accelerate your process development with automated Simple Plex assays for viral titer, process impurity testing, and T cell characterization.
Simple Plex Viral Titer Assays
Accurate detection and quantitative measurement of viral titer in cell cultures is critical for implementation of effective gene transfer treatment strategies. Simple Plex assays are available to quantify viral titers of both lentivirus and adeno-associated virus capsids.
The Simple Plex HEK 293 HCP 3G Assay
The Simple Plex HEK 293 HCP 3G (SPCKB-OT-007066) assay on Ella offers broad detection coverage of the most relevant host cell proteins (HCPs). Ella delivers excellent sensitivity and extended dynamic range to ensure precise sample detectability at each stage of the purification process. The assay is based on the same antibodies currently used in the Cygnus HEK 293 HCP ELISA kit, 3G Resupply 1 (F650S). As a result, you get the same accuracy and consistency you would expect from the Cygnus ELISA, but with an automated, streamlined workflow. The Simple Plex HEK 293 HCP 3G assay is available for purchase directly from Cygnus Technologies.
|Simple Plex HEK 293 HCP 3G Specs|
How Does the Simple Plex HEK 293 HCP 3G Assay Compare with the Cygnus ELISA?
In this application note, we demonstrate assay comparability between the Simple Plex HEK 293 HCP 3G assay and the Cygnus HEK 293 HCP ELISA 3G Resupply 1 ELISA to show that you get the same high-quality data you would expect from the Cygnus ELISA with an automated, streamlined workflow.
Multianalyte Measurements For Immune Cell Characterization
Multianalyte cartridges on Ella allow you to take multiple biomarker measurements in parallel rather than in multiplex. This ensures the highest quality data since all assays are run in a discrete environment. Combine any four analytes from our menu into a custom panel for your immune cells.
Quantify Secreted T Cell Activation Markers in 90 Minutes
Simple Plex assays on the Ella platform offer a rapid and precise assessment of secreted T cell activation markers to boost cell therapy development and manufacturing efficiency. In this application note, we use TcBusterTM, Bio-Techne’s novel non-viral gene delivery technology, to generate CD19 CAR T cells and validate the use of Ella as a T Cell activation marker assay platform. Read on to learn more.
See the full Simple Plex assay menu, learn more about how Ella works or talk to one of our Immunoassay specialists about Cell and Gene Therapy solutions for your lab.
Bio-Techne's Tools For Cell Therapy Development
Bio-Techne offers a diverse portfolio of products and services designed to accelerate and simplify your cell and gene therapy workflow. From GMP proteins and small molecules to state-of-the-art analytical instrumentation, Bio-Techne is committed to providing high-quality innovative solutions. Learn more about Bio-Techne’s cell and gene therapy offerings.
Simple Plex Applications
Quantify cytokine and pro-inflammatory responses to SARS-CoV-2 infection in 90 minutes using an automated, multianalyte format.
Simple Plex assays enable rapid and reproducible quantification of inflammatory responses such as cytokine release syndrome and sepsis.
Measure key neuroscience biomarkers such as neurofilament light (NF-L) and neurofilament heavy (NF-H) in CSF, plasma, and serum.
Cell Therapy Research and Development
Cell Therapy Research and Development
The Simple Plex HEK 293 HCP 3G assay on the Ella platform offers broad detection coverage of this host cell protein (HCP).
With a four-log range of detection, Simple Plex assays have the sensitivity to pick up even very low picogram-per-milliliter levels of endogenous mouse or rat analytes.
Viral Titer Assays
Viral Titer Assays
Get highly reproducible adeno-associated virus and lentivirus titer data with no manual steps in just 90 minutes.
Simple Plex Cartridge Denominations