Simple Plex Human IL-15 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000500
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human IL-15 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-15 assay.
Inter-Assay Precision (Precision between assays) Replicates of each IL-15 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human IL-15.
Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 10 | 9 |
| Mean (pg/mL) | 10.3 | 562 | 10.8 | 540 |
| Standard Deviation | 0.666 | 40.2 | 0.598 | 29.6 |
| CV% | 6.5 | 7.2 | 5.6 | 5.5 |
Recovery for Simple Plex Human IL-15 Cartridge
Recovery for the human IL-15 assays was evaluated at three different spiked concentrations of IL-15 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=6) | 101 | 94-109 |
| EDTA Plasma (n=5) | 93 | 82-106 |
| Heparin Plasma (n=5) | 98 | 83-106 |
| Serum (n=3) | 99 | 95-104 |
Linearity
Samples containing and/or spiked with high concentrations of human IL-15 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human IL-15 Cartridge
Simple Plex Human IL-15 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human IL-15 Assay. Assay range is 0.51-1950 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: IL-15
IL-15 (Interleukin 15) is a cytokine that induces or enhances the differentiation, maintenance, or activation of multiple T cell subsets including NK, NKT, Th17, Treg, and CD8+ memory cells. It also induces dendritic cell differentiation and inflammatory activation, exhibits anti-tumor activity, and inhibits the deposition of lipid in adipocytes. Complexes of IL-15 with cell surface IL-15 R alpha interact with complexes of IL-2 R beta and the Common gamma Chain on adjacent cells. This transpresentation mechanism enables cells to respond to IL-15 even if they do not express IL-15 R alpha. Ligation of membrane-associated IL-15/IL-15 R alpha complexes also induces reverse signaling that promotes activation of the IL-15/IL-15 R alpha expressing cells. The activity of circulating IL-15 is limited by its association with soluble IL-15 R alpha.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional IL-15 Products
Product Documents for Simple Plex Human IL-15 Cartridge
Product Specific Notices for Simple Plex Human IL-15 Cartridge
For research use only