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Ella Automated Immunoassay System

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Ella Automated Immunoassay System
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I need to transfer my immunoassay and get the same results
A Reputation of Quality: The Bio-Techne Brand Story

Ella: Your Next Generation ELISA

Ella's combination of performance and workflow brings your immunoassays to the next level. In just 90 minutes you get highly reproducible validated assay data with no manual steps. The assay performance behind that data includes sub-picogram level sensitivity, 4+ logs of dynamic range and reproducibility that rivals the best laboratory automation. All in a compact package that doesn't break your budget or your lab bench.

ELISA results. One hour.

See how Simple Plex eliminates all the challenges of traditional ELISAs:
  • Save time with automated assays run on Ella in just an hour
  • Get quantitative, reproducible data using 25 µL with a 4-5 log dynamic range
  • Eliminate cross-reactivity by detecting each analyte in separate parallel channels

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Testimonials from your Peers for Simple Plex

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"We needed assays that were not only excellent at conserving our limited sample volumes but would allow us to test a lot of markers quickly and efficiently. The Luminex and Simple Plex assays proved to be the ideal choice in this case, giving us a level of accuracy and reproducibility that we’ve been unable to get with other platforms."

Dr. Melissa Munroe, M.D., Ph.D., Research Associate, Oklahoma Medical Research Foundation (OMRF)

"Simple Plex assays on Ella gave us speed and efficiency so we could complete our assays quickly, and the high quality of the data meant that we could easily see differences between disease state samples and controls. Plus, sample preparation was so simple it reduced chances of errors significantly."

Paulomi Aldo, Research Associate and Reproductive Sciences Core Manager, Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University

"Ella allows for reproducible sample data with minimal sample input. The lower volume usage allows for the expansion of research into novel areas of angiogenesis and inflammation as they pertain to many forms of cancer."

Andrew Nixon Ph.D., M.B.A.,, Associate Professor, Medicine & Director, Duke University

"Ella is the closest I’ve seen to my goal of one day having a Star Trek-like tricorder. I can take just 25 μL of serum, add it to a cartridge, and in 90 minutes have immune profiling data."

E. Scott Halstead, M.D., Ph.D., Associate Professor, Department of Pediatrics, Penn State University College of Medicine

"Ella is a simple and fast immunoassay platform that would be ideal for implementation in higher biosafety facilities, like BSL-3, to study the acute phase of SARS-CoV-2 infection."

Anthony Tanoto Tan, Ph.D., Senior Research Fellow, Duke-NUS Medical School, Singapore

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FAQs for Ella Automated Immunoassay System

  • Where can Certificates of Analysis (CoAs) be found for Simple Plex cartridges and controls?

    Proceed to the Certificate of Analysis Finder Tool on the Bio-Techne.com website here: https://www.bio-techne.com/support/cofa-finder-tool.htmlFrom here, please enter the full catalog number and the lot number in the appropriate fields. 

  • Can expired cartridges be run on the Ella?

    The Ella instrument is not programmed to reject expired cartridges, however Bio-Techne can only support the performance of cartridges within the expiration date.

  • What is the minimum sample volume required for Simple Plex assays?

    A minimum final volume of 50 µL of diluted sample is required per sample inlet. See analyte specific specification sheet for Minimum Required Dilution (MRD). 

  • What is the expiration date of a Simple Plex cartridge?

    Simple Plex cartridges expire one year from the date of manufacture.

  • What is the expiration date of Simple Plex controls?

    Lyophilized controls are stable for up to 12 months from the date of receipt when stored at 2-8 °C. Reconstituted controls are stable for up to 6 months from date of preparation when stored at ≤ -70 °C. Storage at -20 °C is not recommended.

  • Why do Simple Plex controls need to be frozen before use?

    The controls need to be frozen to ensure consistent readings between control aliquots.  Control aliquots used fresh may give different readings in the Simple Plex assays than aliquots that have been exposed to a freeze/thaw cycle.

  • Are controls included when ordering Simple Plex cartridges?

    Simple Plex controls are sold separately. Instructions for use can be found on the control insert and lot-specific CoA. 

  • Are custom Simple Plex assays available for analytes not currently on the menu?

    Custom panels with existing targets can be built using our Simple Plex Panel Builder Tool. Not finding a target you are interested in? Custom options are available for the Ella platform. Please fill out the custom services form and our team will contact you soon. Click Here

  • Does Ella interface with a Laboratory Information Management System (LIMS)? 

    The Ella platform supports integration with Watson LIMS and also includes a Generic LIMS option for use with other LIMS interfaces. Please reference the Ella User Guide for more information regarding specific settings and export instructions.

  • Can users be tracked and use separate log in accounts using the Simple Plex Software?

    After purchase and activation of the 21 CFR Part 11 capabilities, the software can be used to track users and each user will have their own separate log in. Please see the Ella User Guide for more information about the 21 CFR Part 11 capabilities.

  • Are Simple Plex assays correlated to R&D Systems ELISA Assays?  

    Many Simple Plex assays have been correlated to R&D Systems Quantikine ELISAs. Simple Plex assays are not correlated to R&D Systems Luminex or DuoSet assays.

  • Can Simple Plex assays be fully automated with a Liquid Handler? 

    Each of the cartridge inlets are spaced the same as a 96-well ELISA plate, but the dimensions are different for each cartridge format (48-Digoxigenin, 16x4, 32x4, 32x8, or 72x1). If you need more detailed dimensions of the plate to generate your liquid handler settings, please contact Technical Service. It may require some experimentation from the end user to determine optimal settings.

  • Is assay performance comparable in a Single Plex and in a Multianalyte Simple Plex cartridge?

    Yes. The multianalyte cartridges have separate microfluidic chambers for each analyte. These multianalyte assays are not true multiplex assays, but rather 4 single plex assays run in parallel. The antibodies do not interact, so antibody compatibility and cross-reactivity are not a factor in considering whether any two analytes are compatible. In the Multiplex cartridges, up to two analytes share a single channel. Analytes available in the Multiplex cartridge have been fully validated as a panel to ensure that there is not any cross-reactivity or interference, and assay performance is comparable to the same assay run in a Single Plex or Multianalyte cartridge.

  • Can high molecular weight proteins be run in a Simple Plex assay? 

    Simple Plex cartridges do not have any size limitations. The Glass Nano Reactors (GNRs) have an approximate inner diameter of 72 microns, which can accommodate very high molecular weight proteins.

  • Can the assay range change from lot-to-lot in Simple Plex assays?

    The Limits of Quantification for each assay are established during development and do not change lot-to-lot.

  • What is a self-test and how often should it be run? What is a verification cartridge? 

    The Ella includes a verification cartridge that enables the system to perform a self-test that ensures the instrument is running properly. The verification cartridge features a level indicator, a surface to seal Ella’s pneumatic interface, and an auto-fluorescing glass covered surface as an RFU reference. It is recommended that the self-test is run once a week to create a running history of system performance. Whenever handling the verification cartridge, wear gloves to avoid getting it dirty. 

More Information About Ella Automated Immunoassay System

Simple Plex Technology

Ella does immunoassays in a microfluidic Simple Plex cartridge. It's like using a pre-kitted immunoassay, except everything's pre-loaded on the cartridge—even the calibration curve! All you need to do is add your sample and buffers and put the cartridge in Ella.

The immunoassay part works like this: sample runs through a microfluidic channel that binds your protein of interest. Next, Ella washes off unbound analyte and adds a detection reagent. Because each channel has three Glass Nano Reactors (GNRs) coated with a capture antibody, you get triplicate answers for each sample. Answers are then generated from the factory-calibrated standard curve that comes with every cartridge. Sound familiar? It's the same immunoassay you're used to, just without the errors that come with traditional assays.

microfluidics diagram

Simple Plex Applications

sars cov-2 virus

COVID-19 Research

Quantify cytokine and pro-inflammatory responses to SARS-CoV-2 infection in 90 minutes using an automated, multianalyte format.

joint inflammation

Inflammation Research

Simple Plex assays enable rapid and reproducible quantification of inflammatory responses such as cytokine release syndrome and sepsis. 

immune cells attack cancer cell

Cancer Biomarker Research

Simple Plex assays for cancer research have the sensitivity to profile low abundance cancer biomarkers.

neuronal network

Neuroscience Research

Measure key neuroscience biomarkers such as neurofilament light (NF-L) and neurofilament heavy (NF-H) in CSF, plasma, and serum.

Cell and gene therapy

Cell Therapy Research and Development

The Simple Plex HEK 293 HCP 3G assay on the Ella platform offers broad detection coverage of this host cell protein (HCP).

protein bioprocessing workflow

Bioprocess Testing

Iterate your workflow faster with Simple Plex CHO HCP 3G-1 assays for process-related impurity testing.

preclinical scientist pipetting in the lab

Preclinical Research

With a four-log range of detection, Simple Plex assays have the sensitivity to pick up even very low picogram-per-milliliter levels of endogenous mouse or rat analytes.

AAV2 virus for simple plex

Viral Titer Assays

Get highly reproducible adeno-associated virus and lentivirus titer data with no manual steps in just 90 minutes.

Description Single Plex Specification Multianalyte Specification Multiplex Specification Customizable Specification
Sample Volume Required 2.5-25 µL 2.5-25 µL 2.5-25 µL 2.5-25 µL
Glass Nano Reactor CV <4%
Validated Intra-assay CV <10% N/A
Validated Inter-assay CV <12% <15% N/A
Dynamic range 3-4 logs
Sensitivity <1pg/mL (assay dependent)
Assay Runtime 75 Minutes 80 Minutes
Samples per run Up to 72 Up to 32 Up to 32 Up to 48
Plex level 1 Up to 4 Up to 8 1
Results per run Up to 72 Up to 128 Up to 256 Up to 48
Replicates per result Triplicate Triplicate Duplicate Triplicate
Ella Weight 16 kg (35.3 lbs)
Ella Dimensions (closed) 37 cm W x 54 cm D x 26 cm H
Ella Power 100-240 V(AC), 300 VA, 45/65 Hz, Class I Installation Category(Over Voltage) II
Ella Operating temperature 15 to 30 °C (59° to 86°F)
Ella Operating humidity 15-80% relative, non-condensing

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