Simple Plex™ Assays for Bioprocess Testing

To meet increasing demands and safety standards, fast and reproducible detection of host cell proteins (HCPs) is essential during the manufacturing process of a biotherapeutic. Automated Simple Plex™ assays on Ella™ are the combination of sample throughput and hands-off workflow needed to increase your process development bandwidth.

• Ella reduces the manual operation of an immunoassay by 80%, to simply diluting samples and adding them to a cartridge
• With a run time of just 75 minutes, Ella allows your process development to iterate faster
• Simple Plex assays provide sub-picogram level detection, giving you superior assay sensitivity at each stage of the purification process

To bring you the best quality immunoassays, we've partnered with the industry leader in bioprocess impurity testing. Cygnus Technologies provides the highest level of validation and characterization of the antibodies that go into their assays, ensuring that HCP coverage is complete and reproducible. Learn more about Cygnus’ orthogonal characterization, in accordance with standard FDA and ICH guidelines.

The Simple Plex CHO HCP 3G-1 assay (SPCKB-OT-003714) on Ella and Runner software are GMP-compliant and ready for establishment in GMP labs. The assay is based on the same antibodies currently used in the Cygnus CHO HCP ELISA Kit, 3G (F550-1), which utilizes antibodies that have been evaluated for reactivity to more than 1,000 individual HCPs present in CHO strains by state-of-the-art Antibody Affinity Extraction™ and mass spectrometry methods.

The Simple Plex CHO HCP 3G-1 Assay is available for purchase directly from Cygnus Technologies.

Take your CHO HCP measurements to the next level with Ella. In this application note, we demonstrate assay comparability between the Simple Plex CHO HCP 3G-1 assay and the Cygnus 3G ELISA (F550-1) to show that you get the same high-quality data you would expect from the Cygnus ELISA but with an automated, streamlined workflow. Now you can get the best of both worlds by running the Cygnus CHO HCP 3G immunoassay on Ella.