Simple Plex Protein A Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-OT-009204
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Protein A Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each Protein A control was tested multiple times in one Protein A assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Bioprocess Samples
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 29 | 27 |
| Mean (pg/mL) | 216 | 8267 | 167 | 7065 |
| Standard Deviation | 4.60 | 234 | 19.8 | 642 |
| CV% | 2.1 | 2.8 | 11.8 | 9.1 |
Recovery for Simple Plex Protein A Cartridge
Recovery at four different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Bioprocess Samples (n=6) | 97 | 90-103 |
Linearity
Samples containing and/or spiked with high concentrations Protein A were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Protein A Cartridge
Simple Plex Protein A Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Protein A Assay. Assay range is 11.50-17,500 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: Protein A
Protein A is a Staphylococcus aureus cell wall constituent that is characterized by its binding affinity to the Fc portion of some immunoglobulins, especially those of the IgG class. Because of this property, it is often utilized for microscopic visualization of molecules and to purify antisera. Protein A has also been used for the treatment of proteinuria in nephrotic syndrome, and severe autoimmune diseases such as rheumatoid arthritis, coeliac disease, and systemic lupus erythematosis.
Additional Protein A Products
Product Documents for Simple Plex Protein A Cartridge
Product Specific Notices for Simple Plex Protein A Cartridge
For research use only