Simple Plex Endonuclease Cartridge Best Seller

ProteinSimple, part of Bio-Techne | Catalog # SPCKC-OT-008612

Simple Plex Endonuclease assay kit contains cartridge, sample diluent SD19, and wash buffer.
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SPCKA-OT-008612
SPCKC-OT-008612
ST01C-OT-008612
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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Bioprocess Samples (25 µL)

Sensitivity

0.415 pg/mL

Assay Range

1.97-18750 pg/mL (Bioprocess Samples)

Simple Plex Endonuclease Cartridge Summary

Simple Plex assay for the detection of Endonuclease in bioprocess samples. Denarase® and Benzonase® are detectable in this assay. Benzonase® is a registered trademark of Merck KGaA Corp. Denarase® is a registered trademark of c-LEcta GmbH.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Endonuclease Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Bioprocess Samples

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 40 43
Mean (pg/mL) 191 5922 126 5636
Standard Deviation 12.2 194 14.6 576
CV% 6.4 3.3 11.6 10.2

Recovery for Simple Plex Endonuclease Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Bioprocess Samples (n=3) 94 90-97

Linearity

Samples containing and/or spiked with high concentrations Endonuclease were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Endonuclease Assay Linearity

Scientific Data Images for Simple Plex Endonuclease Cartridge

Simple Plex Endonuclease Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Endonuclease Assay. Assay range is 1.97-18,750 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Product Documents for Simple Plex Endonuclease Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Endonuclease Cartridge

For research use only

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