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Simple Plex™ Assays for Viral Titer Quantification

Fast and reproducible viral capsid measurement is essential for those focused on gene transfer, as part of a cell and gene therapy workflow. While traditional plate-based and dot blot assays can be useful, they are often complex, time-consuming, and non-standardized. See how Simple Plex assays on the EllaTM platform are used for viral titer quantification. Next, meet Ella and request pricing. Finally, choose the Simple Plex Assay that best meets your needs.

Table of Contents

AAV PROGEN
Automated ELISAs on immunoassay platform Ella applications include viral titer assays and cytokine release assays

Automated Simple Plex assays on the Ella platform offer a fast, sensitive, and reproducible method for reliable viral capsid measurement. Simple Plex assays are available to quantify the viral titer of both lentivirus and adeno-associated virus capsids.

  • Measure viral titer across a broad dynamic range
  • Get triplicate data results with just 25 µL or less of sample volume
  • Streamline workflow with only 15 minutes of hands-on assay prep time
  • Achieve the same reproducible answers across multiple users and multiple sites
  • Get highly reproducible validated data with no manual steps in just 90 minutes

Scaling analytics for cell and gene therapy with Ella

Scaling Analytics for Cell and Gene Therapy With Ella

Watch how Dr. Kiren Baines, a bioprocessing scientist from eXmoor, uses Ella to scale analytics for AAV process development.

Fast and Reproducible AAV Vector Titration

To meet increased throughput and safety demands, investigators are seeking more automated analytical solutions for assessment of viral vector identity, purity, and stability. Simple Plex AAV assays on the Ella platform provide an accurate and efficient method for measuring the AAV capsid titer. The assay uses the recombinant AAV antibodies from industry leader PROGEN to quantify assembled AAV capsids.

Combining the proven specificity of PROGEN AAV antibodies with the convenient workflow and robustness of the Ella platform, Simple Plex AAV viral titer assays:

  • Offer a broad viral detection range combined with hands-free automation to accelerate AAV viral capsid quantification
  • Use factory calibrated assay cartridges, which minimize setup time and reduce user-dependent variability while reducing the need for sample retests
  • Rely on the innovative Ella platform to perform every step of the immunoassay automatically, from wash steps to final
  • Provide a robust alternative to the conventional ELISA or dot blot approaches
  • Deliver superior assay sensitivity, helping to ensure data quality and consistency
Multiplex immunoassays for biomarker research

Serotype PROGEN Monoclonal Antibody Simple Plex Assay LLOQ (capsids/mL) Simple Plex Assay ULOQ (capsids/mL)
AAV1 ADK1 2.62 X 106 1.0 X 1010
AAV2 A20 3.41 X 106 1.3 X 1010
AAV6 ADK1 3.04 X 106 1.16 X 1010
AAV8 ADK8 2.47 X106 9.428 X 1010

First page of the Simple Plex AAV application note

Comparing Simple Plex AAV Assays With the PROGEN AAV ELISAs

In this application note, we demonstrate assay comparability between the Simple Plex AAV assays and the PROGEN AAV Titration ELISAs to show that you get the same high-quality data you would expect from the PROGEN ELISA with an automated, streamlined workflow.

 

 

Creating Lentiviral Vectors With Fast, Reliable Viral Titration

With the growing use of lentiviral vectors derived from HIV-1 in gene transfer workflows, researchers need fast, reliable viral titer quantification during process development. The 3-to-6-hour timeframe required to run a standard ELISA is a challenge for operators who increasingly require results quickly to inform real-time decision-making.

The Simple Plex assay for the detection of human HIV-1 Gag p24 combines high throughput with superior accuracy and precision to enable rapid quantification of lentiviral capsid proteins throughout different stages of cell and vector manufacturing. The automated, reproducible nature of the Simple Plex p24 assay helps eliminate user error, making it an ideal tool in good manufacturing practice (GMP) environments where quality, standardization of process, assay transfer, and scalability are highly desirable.

View HIV-1 GAG p24 Assay

Bio-Techne's Tools For Cell And Gene Therapy

Bio-Techne offers a diverse portfolio of products and services designed to accelerate and simplify your cell and gene therapy workflow. From GMP proteins and small molecules to state-of-the-art analytical instrumentation, Bio-Techne is committed to providing high quality innovative solutions. Learn more about Bio-Techne’s cell and gene therapy offerings.

Learn About Bio-Techne Cell & Gene Therapy Solutions

Simple Plex Applications

sars cov-2 virus

COVID-19 Research

Quantify cytokine and pro-inflammatory responses to SARS-CoV-2 infection in 90 minutes using an automated, multianalyte format.

joint inflammation

Inflammation Research

Simple Plex assays enable rapid and reproducible quantification of inflammatory responses such as cytokine release syndrome and sepsis. 

immune cells attack cancer cell

Cancer Biomarker Research

Simple Plex assays for cancer research have the sensitivity to profile low abundance cancer biomarkers.

neuronal network

Neuroscience Research

Measure key neuroscience biomarkers such as neurofilament light (NF-L) and neurofilament heavy (NF-H) in CSF, plasma, and serum.

Cell and gene therapy

Cell Therapy Research and Development

The Simple Plex HEK 293 HCP 3G assay on the Ella platform offers broad detection coverage of this host cell protein (HCP).

protein bioprocessing workflow

Bioprocess Testing

Iterate your workflow faster with Simple Plex CHO HCP 3G-1 assays for process-related impurity testing.

preclinical scientist pipetting in the lab

Preclinical Research

With a four-log range of detection, Simple Plex assays have the sensitivity to pick up even very low picogram-per-milliliter levels of endogenous mouse or rat analytes.

AAV2 virus for simple plex

Viral Titer Assays

Get highly reproducible adeno-associated virus and lentivirus titer data with no manual steps in just 90 minutes.

Simple Plex Cartridge Denominations

Automated ELISA platform assay options including singleplex, multianalyte and multiplex assay cartridges

 

 

  16x1 32x1 16x2 16x3 16x4 32x2 72x1 32x3 32x4 32x5 32x6 32x7 32x8
Samples 16 32 16 16 16 32 72 32 32 32 32 32 32
Plex 1 1 2 3 4 2 1 3 4 5 6 7 8
Replicates 3 3 3 3 3 3 3 3 3 2 2 2 2
Reportable Results 16 32 32 48 64 64 72 96 128 160 192 224 256