Where can Certificates of Analysis (CoAs) be found for Simple Plex cartridges and controls?

Proceed to the Certificate of Analysis Finder Tool on the Bio-Techne.com website here: https://www.bio-techne.com/support/cofa-finder-tool.htmlFrom here, please enter the full catalog number and the lot number in the appropriate fields.

Can expired cartridges be run on the Ella?

The Ella instrument is not programmed to reject expired cartridges, however Bio-Techne can only support the performance of cartridges within the expiration date.

What is the minimum sample volume required for Simple Plex assays?

A minimum final volume of 50 µL of diluted sample is required per sample inlet. See analyte specific specification sheet for Minimum Required Dilution (MRD).

What is the expiration date of a Simple Plex cartridge?

Simple Plex cartridges expire one year from the date of manufacture.

What is the expiration date of Simple Plex controls?

Lyophilized controls are stable for up to 12 months from the date of receipt when stored at 2-8 °C. Reconstituted controls are stable for up to 6 months from date of preparation when stored at ≤ -70 °C. Storage at -20 °C is not recommended.

Why do Simple Plex controls need to be frozen before use?

The controls need to be frozen to ensure consistent readings between control aliquots. Control aliquots used fresh may give different readings in the Simple Plex assays than aliquots that have been exposed to a freeze/thaw cycle.

Are controls included when ordering Simple Plex cartridges?

Simple Plex controls are sold separately. Instructions for use can be found on the control insert and lot-specific CoA.

Are custom Simple Plex assays available for analytes not currently on the menu?

Custom panels with existing targets can be built using our Simple Plex Panel Builder Tool. Not finding a target you are interested in? Custom options are available for the Ella platform. Please fill out the custom services form and our team will contact you soon. Click Here

Does Ella interface with a Laboratory Information Management System (LIMS)?

The Ella platform supports integration with Watson LIMS and also includes a Generic LIMS option for use with other LIMS interfaces. Please reference the Ella User Guide for more information regarding specific settings and export instructions.

Can users be tracked and use separate log in accounts using the Simple Plex Software?

After purchase and activation of the 21 CFR Part 11 capabilities, the software can be used to track users and each user will have their own separate log in. Please see the Ella User Guide for more information about the 21 CFR Part 11 capabilities.

Are Simple Plex assays correlated to R&D Systems ELISA Assays?

Many Simple Plex assays have been correlated to R&D Systems Quantikine ELISAs. Simple Plex assays are not correlated to R&D Systems Luminex or DuoSet assays.

Can Simple Plex assays be fully automated with a Liquid Handler?

Each of the cartridge inlets are spaced the same as a 96-well ELISA plate, but the dimensions are different for each cartridge format (48-Digoxigenin, 16x4, 32x4, 32x8, or 72x1). If you need more detailed dimensions of the plate to generate your liquid handler settings, please contact Technical Service. It may require some experimentation from the end user to determine optimal settings.

Is assay performance comparable in a Single Plex and in a Multianalyte Simple Plex cartridge?

Yes. The multianalyte cartridges have separate microfluidic chambers for each analyte. These multianalyte assays are not true multiplex assays, but rather 4 single plex assays run in parallel. The antibodies do not interact, so antibody compatibility and cross-reactivity are not a factor in considering whether any two analytes are compatible. In the Multiplex cartridges, up to two analytes share a single channel. Analytes available in the Multiplex cartridge have been fully validated as a panel to ensure that there is not any cross-reactivity or interference, and assay performance is comparable to the same assay run in a Single Plex or Multianalyte cartridge.

Can high molecular weight proteins be run in a Simple Plex assay?

Simple Plex cartridges do not have any size limitations. The Glass Nano Reactors (GNRs) have an approximate inner diameter of 72 microns, which can accommodate very high molecular weight proteins.

Can the assay range change from lot-to-lot in Simple Plex assays?

The Limits of Quantification for each assay are established during development and do not change lot-to-lot.

What is a self-test and how often should it be run? What is a verification cartridge?

The Ella includes a verification cartridge that enables the system to perform a self-test that ensures the instrument is running properly. The verification cartridge features a level indicator, a surface to seal Ella’s pneumatic interface, and an auto-fluorescing glass covered surface as an RFU reference. It is recommended that the self-test is run once a week to create a running history of system performance. Whenever handling the verification cartridge, wear gloves to avoid getting it dirty.

Ella Automated Immunoassay System Best Seller

ProteinSimple, part of Bio-Techne | Catalog # 600-100

(2)

Citations (2)

Product Documents

Ella: Your Next Generation ELISA

Ella's combination of performance and automated ELISA workflow brings your immunoassays to the next level. In less than 90 minutes you get highly reproducible validated assay data and consistent biomarker detection with no manual steps. The assay performance behind that data includes sub-picogram level sensitivity, 4+ logs of dynamic range and precision that rivals the best laboratory automation. All in a compact package that doesn't break your budget and fits on your benchtop.

Request Information

An Automated Benchtop ELISA Platform

See how Simple Plex eliminates all the challenges of traditional ELISAs:

Save time with automated assays run on Ella with built in standard curves and results in less than 90 minutes
Get quantitative, reproducible data using just 25 µL of sample with up to 5 logs of dynamic range
Eliminate cross-reactivity by detecting each analyte in separate parallel channels

Instrument Service & Support Plans

At Bio-Techne, we know that reliable instruments are the backbone of productive research. Our service and support plans are built to give you confidence, continuity, and control—whether you need complete coverage or selective, budget-friendly support. We offer a full suite of service plans designed to protect your investment, minimize downtime, and maintain peak performance beyond the instrument warranty period. Each tier includes an annual preventive maintenance visit to safeguard performance, while our experienced technicians ensure issues are resolved quickly and effectively. The included annual preventive maintenance inspection ensures your instrument is running at peak performance and up to manufacturer specification for reliable and trustworthy results.

With flexible options designed for academic, government, and industry labs alike, our plans protect your investment and help you keep your work on track.

View Brochure

About Ella Automated Immunoassay System
Citations (2) / Reviews (2) / Testimonials (10) / FAQs

How Can Ella Automated Immunoassay System Help You?

	I want to spend less hands-on time doing the immunoassay You're in a race to make that next discovery, and Ella can help you get there faster. There's no need to split your time between running assays in the lab and analyzing answers at your desk. Simply add your sample and buffers to the Simple Plex cartridge, put it in Ella and start your run. There's only 10-15 minutes of hands-on assay prep time, and she gives you fully analyzed answers in an hour!
	I need to transfer my immunoassay and get the same results When it's time to develop and transfer your immunoassay to other labs, sites or a contract research organization, Ella makes it easy. Because Simple Plex assays are precisely controlled in the cartridge, there's no chance for pipetting errors. You'll get the same reproducible answers across multiple users and multiple sites. Simply put, you'll get consistent answers anywhere, by anyone on any Ella.

Testimonials from your Peers for Simple Plex

Showing 1 - 5 of 10 testimonials Showing All

"With Ella, we can worry less about manpower and time constraints. It’s convenient, quick, and automated—perfect for pediatric studies with limited sample volume."

Fast and Efficient: How the Ella™ System Is Helping to Simplify Pediatric Vaccine Research

Dr. Zhong Youjia, Assistant Professor, Department of Paediatrics, Yong Loo Lin School of Medicine, National University of Singapore

"Ella’s sensitive and tractable workflow is enabling us to tackle large datasets not previously possible with standard ELISA kits. It has enabled us to more quickly and efficiently discover novel connections between hepcidin and inflammation in the context of chronic disease."

Fast and Efficient Data Analysis: Advancing Our Understanding of Iron-Related Disorders With Ella

Samira Lakhal-Littleton, BSc DPhil, Associate Professor of Cell Physiology, University of Oxford

"We needed assays that were not only excellent at conserving our limited sample volumes but would allow us to test a lot of markers quickly and efficiently. The Luminex and Simple Plex assays proved to be the ideal choice in this case, giving us a level of accuracy and reproducibility that we’ve been unable to get with other platforms."

Measuring Immune Response to Predict Changes in Autoimmune Disease Activity

Dr. Melissa Munroe, M.D., Ph.D., Research Associate, Oklahoma Medical Research Foundation (OMRF)

"Simple Plex assays on Ella gave us speed and efficiency so we could complete our assays quickly, and the high quality of the data meant that we could easily see differences between disease state samples and controls. Plus, sample preparation was so simple it reduced chances of errors significantly."

Paulomi Fast-tracks Detection of Viral Responses During Pregnancy with Ella

Paulomi Aldo, Research Associate and Reproductive Sciences Core Manager, Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University

"Ella allows for reproducible sample data with minimal sample input. The lower volume usage allows for the expansion of research into novel areas of angiogenesis and inflammation as they pertain to many forms of cancer."

Angiogenesis and the Search for Novel Biomarkers

Andrew Nixon Ph.D., M.B.A.,, Associate Professor, Medicine & Director, Duke University

"Ella is the closest I’ve seen to my goal of one day having a Star Trek-like tricorder. I can take just 25 μL of serum, add it to a cartridge, and in 90 minutes have immune profiling data."

Simplifying and Expediting Inflammatory Biomarker Research with Ella

E. Scott Halstead, M.D., Ph.D., Associate Professor, Department of Pediatrics, Penn State University College of Medicine

"Ella is a simple and fast immunoassay platform that would be ideal for implementation in higher biosafety facilities, like BSL-3, to study the acute phase of SARS-CoV-2 infection."

Characterizing T-cell Immune Responses Against SARS-CoV-2 with Ella

Anthony Tanoto Tan, Ph.D., Senior Research Fellow, Duke-NUS Medical School, Singapore

"There is enormous potential value for applying Ella in a personalized medicine setting."

Ella Promises Better Clinical Care for Acute Respiratory Failure and Sepsis

Michael Matthay, M.D., Ph.D., Professor of Medicine, University of California San Francisco

"A major advantage of Ella is that it’s very easy to use with virtually no maintenance, and it’s really fast. Simply dilute your sample and you’re ready to go—without the need for manual preparations that can take time and add user variability."

Profiling Host Responses in COVID-19 Infections: Accelerating Biomarker Research with Ella

Dr. Jesús F Bermejo-Martín, Principal Investigator, Group for Biomedical Research in Respiratory Infection and Sepsis (BioSepsis) at the Institute of Biomedical Research of Salamanca (IBSAL)/Hospital Universitario Río Hortega de Valladolid

"Ella allows us to rapidly quantify biomarkers from hundreds of cryopreserved samples and correlate them to specific outcomes. Now we’re ready to validate that what we identified in frozen plasma samples correlates to fresh blood samples taken at the point-of-care. Ella’s simplicity and reproducibility will allow us to reliably test samples across different sites, enhancing triage especially in remote locations of developing countries where access to trained healthcare professionals is unlikely."

Dr. Leligdowicz Speeds Up the Transition of Pediatric Sepsis Biomarkers from Validation to the Clinic with Ella

Aleks Leligdowicz, M.D./Ph.D., Department of Medicine, University of Toronto

Showing 1 - 5 of 10 testimonials Showing All

FAQs for Ella Automated Immunoassay System

More Information About Ella Automated Immunoassay System

How Does Ella Automated Immunoassay System Work?

Simple Plex Technology

Ella does immunoassays in a microfluidic Simple Plex cartridge. It's like using a pre-kitted immunoassay, except everything's pre-loaded on the cartridge—even the calibration curve! All you need to do is add your sample and buffers and put the cartridge in Ella.

The immunoassay part works like this: sample runs through a microfluidic channel that binds your protein of interest. Next, Ella washes off unbound analyte and adds a detection reagent. Because each channel has three Glass Nano Reactors (GNRs) coated with a capture antibody, you get triplicate answers for each sample. Answers are then generated from the factory-calibrated standard curve that comes with every cartridge. Sound familiar? It's the same immunoassay you're used to, just without the errors that come with traditional assays.

Applications

Simple Plex Applications

Products Compatible with Ella Automated Immunoassay System

Specifications

Description	Single Plex Specification	Multianalyte Specification	Multiplex Specification	Customizable Specification
Sample Volume Required	2.5-25 µL	2.5-25 µL	2.5-25 µL	2.5-25 µL
Dynamic range	3-4 logs
Sensitivity	<1pg/mL (assay dependent)
Assay Runtime	75 Minutes			80 Minutes
Samples per run	Up to 72	Up to 32	Up to 32	Up to 48
Plex level	1	Up to 4	Up to 8	1
Results per run	Up to 72	Up to 128	Up to 256	Up to 48
Replicates per result	Triplicate	Triplicate	Duplicate	Triplicate
Ella Weight	16 kg (35.3 lbs)
Ella Dimensions (closed)	37 cm W x 54 cm D x 26 cm H
Ella Power	100-240 V(AC), 300 VA, 45/65 Hz, Class I Installation Category(Over Voltage) II
Ella Operating temperature	15 to 30 °C (59° to 86°F)
Ella Operating humidity	15-80% relative, non-condensing

Product Documents for Ella Automated Immunoassay System

Installation Guide

Download W-WI-0001 A Interconnections Ella to All-in-one PC

Protocols & Manuals

Download W-DOC-0009 C Ella User Guide

Download Declaration of Conformity Simple Plex Ella D45-0001-001 Rev C

Ella Automated Immunoassay System Best Seller

ProteinSimple, part of Bio-Techne | Catalog # 600-100

Ella: Your Next Generation ELISA

An Automated Benchtop ELISA Platform

Instrument Service & Support Plans

How Can Ella Automated Immunoassay System Help You?

I want to spend less hands-on time doing the immunoassay

I need to transfer my immunoassay and get the same results

Testimonials from your Peers for Simple Plex

FAQs for Ella Automated Immunoassay System

More Information About Ella Automated Immunoassay System

How Does Ella Automated Immunoassay System Work?

Simple Plex Technology

Applications

Simple Plex Applications

COVID-19 Research

COVID-19 Research

Inflammation Research

Inflammation Research

Cancer Biomarker Research

Cancer Biomarker Research

Neuroscience Research

Neuroscience Research

Cell Therapy Research and Development

Cell Therapy Research and Development

Bioprocess Testing

Bioprocess Testing

Preclinical Research