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Simple Plex™ Assays for Neuroscience Biomarker Research

First, see how Simple Plex assays on the EllaTM platform are used for Neuroscience research. Next, meet Ella and request pricing. Finally, choose the Simple Plex Assay that best meets your needs.

Table of Contents

Sensitive Data For Neuroscience Biomarker Research

Simple Plex™ assays on Ella™ give you the combination of sensitivity and reproducibility you need to advance your neuroscience biomarker research.

Simple Plex assays provide high-quality data on low abundance biomarkers in serum, plasma, and cerebrospinal fluid. Ella provides a fully integrated and automated workflow to give you data in just 90 minutes.

With Ella's automation, you also get an extremely high level of reproducibility and reliability. Simple Plex assays reduce manual operation and provide a rock-solid platform for your research.

Developing an NFL Assay
Dr. Yoav Noam discusses NF-L Assay Development

Powered By R&D Systems, A Bio-Techne Brand

With immunoassays developed by industry leader R&D Systems, Ella gives you the data you need to power your neuroscience research with a high level of automation, validation, and reliability.


Neurofilament Light (NF-L) in Serum and Plasma

Neurofilament light chain has been implicated as an important biomarker for a number of neurological disorders. The Simple Plex neurofilament light assay can quantify this biomarker in serum and plasma samples to provide highly robust and reproducible data.

The Simple Plex Assay Correlates with the Uman Diagnostics ELISA


Serum and plasma NF-L levels measured using the Simple Plex Assay correlate with those quantified by the Uman Diagnostics ELISA. Samples were prepared according to the recommendations of the respective kit manufacturer. Levels of NF-L were measured within the linear range of each assay. The dilution factors were used to back-calculate the original concentration in the samples. These values were then plotted against each other, and a linear curve fitting algorithm was applied.


The Simple Plex NF-L Assay is highly correlated with the Uman ELISA
 The Simple Plex NF-L assay has a mean CV of 9.7%

The Simple Plex NF-L Assay Has Excellent Inter-Assay Precision


The same plasma and serum samples, obtained from healthy donors, were prepared according to the Simple Plex NF-L Assay instructions and measured at three different sites. The mean sample coefficient of variation (CV) was less than 10%.

NF-L is Readily Detectable in CSF, Plasma and Serum


The ability to detect neurofilament light in the blood is important to allow minimally-invasive diagnosis and monitoring of disease progression. NF-L is readily detectable in cerebrospinal fluid (CSF), serum, EDTA plasma, and Heparin plasma. Serum and CSF samples are 100% detectable. Plasma samples are 90% detectable.




The Simple Plex assay detects NF-L in Serum, EDTA Plasma, Heparin Plasma and CSF
Simple plex neurofilament light chain assay data showing NF-L plasma levels in controls and ALS patients

The Simple Plex Assay Detects Elevated NF-L in Serum From ALS Patients


ALS patient samples were used as a model system to quantify NF-L levels since previous reports have established elevated NF-L levels in ALS serum. Healthy samples were analyzed on a 72x1 Simple Plex NF-L Cartridge and increased NF-L levels were observed in the ALS samples. Bars represent the median NF-L level ± the interquartile range. The statistical difference was evaluated using the nonparametric Mann-Whitney test with 19 to 20 samples per group.



Peer-Reviewed Publications That Feature the Simple Plex NF-L Assay

Highly Sensitive and Reproducible NF-L Measurements

With the Simple Plex NF-L assay on Ella you get measurements on an automated, robust and reproducible immunoassay platform. In our application note we demonstrate how Ella provides highly reproducible NF-L measurements even at the low levels detected in serum and plasma. Learn how to bring your NF-L research to the next level with Ella.

Download the NF-L App Note



NF-L and NF-H as Important Indicators of Neurodegeneration and Preeclampsia

Neurofilament heavy and light chains are associated with a number of pathological conditions, and both individually and in conjunction are promising better diagnosis and monitoring of a number of diseases. Learn how researchers at Beth Israel Deaconess Medical Center, Harvard Medical School, and Cedars-Sinai in collaboration with Bio-Techne are exploring the promise of NF-L and NF-H as biomarkers. Download our poster on NF-L and NF-H as emerging biomarkers.

Download the NF-L/NF-H Poster



Biomarkers Validated for Cerebrospinal Fluid

Need better biomarker data from your cerebrospinal fluid (CSF) samples? We've got neuroscience biomarker assays that have been fully validated for measurement in CSF. Have precious samples and need to measure multiple biomarkers? Talk to our specialists about creating a custom panel for your research.

Neuroscience Biomarkers Validated for CSF
BAFF Fractalkine IL-15 TACI
CD21 ICAM-1 Lipo-2 TNFR1
CD27 IFNg 3rd Gen NF-H TREM-2
CXCL10 IL-6 2nd Gen Osteopontin 2nd Gen  
Ferritin IL-8 Procalcitonin



Simple Plex Neuroscience Biomarker Research in Literature

Learn how researchers are using neurofilament and neuroinflammation biomarker assays on Ella in their research.

Simple Plex Applications

sars cov-2 virus

COVID-19 Research

Quantify cytokine and pro-inflammatory responses to SARS-CoV-2 infection in 90 minutes using an automated, multianalyte format.

joint inflammation

Inflammation Research

Simple Plex assays enable rapid and reproducible quantification of inflammatory responses such as cytokine release syndrome and sepsis. 

immune cells attack cancer cell

Cancer Biomarker Research

Simple Plex assays for cancer research have the sensitivity to profile low abundance cancer biomarkers.

neuronal network

Neuroscience Research

Measure key neuroscience biomarkers such as neurofilament light (NF-L) and neurofilament heavy (NF-H) in CSF, plasma, and serum.

Cell and gene therapy

Cell Therapy Research and Development

The Simple Plex HEK 293 HCP 3G assay on the Ella platform offers broad detection coverage of this host cell protein (HCP).

protein bioprocessing workflow

Bioprocess Testing

Iterate your workflow faster with Simple Plex CHO HCP 3G-1 assays for process-related impurity testing.

preclinical scientist pipetting in the lab

Preclinical Research

With a four-log range of detection, Simple Plex assays have the sensitivity to pick up even very low picogram-per-milliliter levels of endogenous mouse or rat analytes.

AAV2 virus for simple plex

Viral Titer Assays

Get highly reproducible adeno-associated virus and lentivirus titer data with no manual steps in just 90 minutes.

Simple Plex Cartridge Denominations

Automated ELISA platform assay options including singleplex, multianalyte and multiplex assay cartridges



  16x1 32x1 16x2 16x3 16x4 32x2 72x1 32x3 32x4 32x5 32x6 32x7 32x8
Samples 16 32 16 16 16 32 72 32 32 32 32 32 32
Plex 1 1 2 3 4 2 1 3 4 5 6 7 8
Replicates 3 3 3 3 3 3 3 3 3 2 2 2 2
Reportable Results 16 32 32 48 64 64 72 96 128 160 192 224 256