Simple Plex Human alpha 2-Macroglobulin Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-008932
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human alpha 2-Macroglobulin Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Saliva, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 64 | 68 |
| Mean (pg/mL) | 515 | 40826 | 766 | 38297 |
| Standard Deviation | 29.7 | 3940 | 85.8 | 4381 |
| CV% | 5.8 | 9.7 | 11.2 | 11.4 |
Recovery for Simple Plex Human alpha 2-Macroglobulin Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 101 | 98-104 |
Linearity
Samples containing and/or spiked with high concentrations of human alpha2-Macroglobulin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human alpha 2-Macroglobulin Cartridge
Human alpha 2-Macroglobulin Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: alpha 2-Macroglobulin
Alpha 2-Macroglobulin (A2M) is an abundant plasma glycoprotein that has structural similarity to other A2M family members including complement components C3, C4 and C5. A2M is a tetramer (720-kDa) of 4 identical subunits (180-kDa) formed by two disulfide bond-linked dimers. It is a general and irreversible protease inhibitor capable of inhibiting all four classes of proteases by a unique trapping mechanism. A2M also binds many cytokines and growth factors. In the brain, A2M binds soluble beta Amyloid. A2M is recognized as a late onset Alzheimers disease gene.
Additional alpha 2-Macroglobulin Products
Product Documents for Simple Plex Human alpha 2-Macroglobulin Cartridge
Product Specific Notices for Simple Plex Human alpha 2-Macroglobulin Cartridge
For research use only