Do the AAV assays recognize fully-assembled capsids?

Yes, these assays detect fully-assembled capsids. Non-assembled protein or partially assembled capsids will not be detected.

Do the AAV assays recognize both natural and recombinant AAV?

Yes, the AAV assays detect both natural and recombinant AAV.

Does the AAV2 assay recognize other AAV serotypes?

This assay recognizes intact AAV2 capsids. The AAV2 antibody (A20R) used in this assay cross-reacts with AAV3.

Do the Simple Plex AAV assays distinguish between full and empty capsids?

No, the AAV assays do not distinguish between full and empty capsids. Complementary methods are required to distinguish full and empty capsids.

What diluent is provided with the AAV assays?

The AAV assays are supplied with SD19 concentrate. The concentrate should be diluted 1:5 in deionized or dilstilled water prior to use.

What is the minimum required dilution (MRD) for bio-process samples?

All samples require a minimum 2-fold dilution for use in Simple Plex assays, but further dilution may be needed for optimal performace. An appropriate dilution factor for each process matrix should be determined experimentally by assessment of sample linearity and spike recovery.

Simple Plex AAV2 Cartridge Best Seller

Name: Simple Plex AAV2 Cartridge
Brand: ProteinSimple
SKU: SPCKB-OT-005905

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-OT-005905

Product Datasheet / COA / SDS

Simple Plex AAV2 assay kit contains cartridge, sample diluent SD19, and wash buffer.

Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Bioprocess Samples (25 uL)
Sensitivity:	0.9 10^6 capsids/mL
Assay Range:	3.41 - 13,000 10^6 capsids/mL
Simple Plex AAV2 Cartridge Summary:	Simple Plex assay for the detection of Adeno-Associated Virus type 2 (AAV2) intact capsids in bioprocess samples. This assay uses PROGEN's AAV2 (A20R) antibody. Sensitivity: 0.9 x 10⁶ capsids/mL Range: 3.41 x 10⁶ - 1.3 x 10¹⁰ capsids/mL

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Technical Data
Citations / FAQs

Product Specifications for Simple Plex AAV2 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Viral
Specificity	This assay recognizes intact AAV2 capsids. The AAV2 antibody (A20R) used in this assay cross-reacts with AAV3.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Bioprocess Samples

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	58	56
Mean (10<sup>6</sup> capsids/mL)	40	2103	42.2	2045
Standard Deviation	3.5	55.3	4.7	158
CV%	8.8	2.6	11.2	7.7

Linearity

Samples containing and/or spiked with high concentrations of AAV2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex AAV2 Cartridge

	AAV2 Simple Plex Assay

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: AAV2

Item	Value
Long Name	Adeno-associated Virus 3
Alternate Names	AAV3, Adeno-associated Virus 2, Coat protein, capsid, major coat protein

FAQs for Simple Plex AAV2 Cartridge

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Product Documents for Simple Plex AAV2 Cartridge

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Simple Plex AAV2 Cartridge Best Seller