Simple Plex Human IL-2 Cartridge Best Seller
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000295
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human IL-2 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-2 assay.
Inter-Assay Precision (Precision between assays) Replicates of each IL-2 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human IL-2.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 8 | 8 |
| Mean (pg/mL) | 15.0 | 648 | 13.0 | 608 |
| Standard Deviation | 0.80 | 32.7 | 1.13 | 34.0 |
| CV% | 5.3 | 5.1 | 8.7 | 5.6 |
Recovery for Simple Plex Human IL-2 Cartridge
Recovery for the human IL-2 assays was evaluated at three different spiked concentrations of IL-2 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 86 | 69-100 |
| Heparin Plasma (n=4) | 90 | 70-103 |
| Serum (n=4) | 92 | 69-102 |
Linearity
Samples containing and/or spiked with high concentrations of human IL-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human IL-2 Cartridge
Simple Plex Human IL-2 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human IL-2 Assay. Assay range is 0.54-2050 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: IL-2
IL-2 (Interleukin-2) is a critical cytokine in T cell biology. It promotes T cell activation and expansion, the development, maintenance and function of regulatory T cells (Treg), and the differentiation of CD8+ T cells into terminal effector cells and memory cells. IL-2 signals through a receptor complex composed of CD25/IL-2 R alpha, IL-2 R beta, and the Common gamma Chain (gamma c). IL-2 R beta is also a component of the IL-15 receptor complex. Gamma c is also a signaling subunit in the receptors for IL-4, -7, -9, -15, and -21.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional IL-2 Products
Product Documents for Simple Plex Human IL-2 Cartridge
Product Specific Notices for Simple Plex Human IL-2 Cartridge
For research use only