Simple Plex Human Granzyme B Cartridge Best Seller
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000496
Need a different panel or sample type? Customize your cartridge here.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human Granzyme B Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Granzyme B assay.
Inter-Assay Precision (Precision between assays) Replicates of each Granzyme B control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Granzyme B.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 20 | 19 |
| Mean (pg/mL) | 28.0 | 1357 | 28.7 | 1377 |
| Standard Deviation | 1.26 | 47.1 | 2.87 | 143 |
| CV% | 4.5 | 3.5 | 10.0 | 10.4 |
Recovery for Simple Plex Human Granzyme B Cartridge
Recovery for the human Granzyme B assays was evaluated at three different spiked concentrations of Granzyme B protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 103 | 91-117 |
| Heparin Plasma (n=4) | 95 | 86-107 |
| Serum (n=4) | 105 | 91-119 |
Linearity
Samples containing and/or spiked with high concentra- tions of human Granyme B were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human Granzyme B Cartridge
Simple Plex Human Granzyme B Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Granzyme B Assay. Assay range is 1.31-5000 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: Granzyme B
Granzyme family serine proteases are stored in the granules of cytotoxic T lymphocytes and natural killer cells. Granzymes contain one S1 protease domain and are synthesized as preproproteins. Granzymes are released toward pathogen infected or transformed target cells during cellular immune reactions. Target cell entry by granzymes is through perforin channels. Granzymes trigger apoptosis by caspase-dependent and -independent mechanisms. There are five granzymes (A, B, G, H and K) in human and many more in mouse and rat.
Alternate Names
Gene Symbol
Additional Granzyme B Products
Product Documents for Simple Plex Human Granzyme B Cartridge
Product Specific Notices for Simple Plex Human Granzyme B Cartridge
For research use only