Simple Plex HIV-1 p24 2nd Gen Cartridge Best Seller
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-CS-009216
Need a different panel or sample type? Customize your cartridge here.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex HIV-1 p24 2nd Gen Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 29 | 29 |
| Mean (µL) | 782 | 36099 | 856 | 37492 |
| Standard Deviation | 38.0 | 2120 | 93.1 | 2511 |
| CV% | 4.9 | 5.9 | 10.9 | 6.7 |
Recovery for Simple Plex HIV-1 p24 2nd Gen Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media Pretreated (n=7) | 101 | 91-111 |
| Cell Culture Media Untreated (n=7) | 104 | 90-115 |
Linearity
Samples containing and/or spiked with high concentrations of HIV-1 p24 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex HIV-1 p24 2nd Gen Cartridge
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex HIV-1 p24 Assay. Assay range is 11.7-112,000 pg/mL.
Preparation and Storage
Shipping
Stability & Storage
Background: HIV-1 Gag p24
The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional HIV-1 Gag p24 Products
Product Documents for Simple Plex HIV-1 p24 2nd Gen Cartridge
Product Specific Notices for Simple Plex HIV-1 p24 2nd Gen Cartridge
For research use only