Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human beta-1,3-N-acetylglucosaminyltransferase 2/B3GNT2
Lys29-Cys397
Accession # Q9NY97

Specificity

Detects human and rat beta-1,3-N-acetylglucosaminyltransferase 2/B3GNT2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Rat beta-1,3-N-acetylglucosaminyl-transferase 2/B3GNT2 Antibody

Detection of Human and Rat beta-1,3-N-acetylglucosaminyl-transferase 2/B3GNT2 antibody by Western Blot.

Detection of Human and Rat beta-1,3-N-acetylglucosaminyl-transferase 2/B3GNT2 by Western Blot.

Western blot shows lysates of human brain tissue and rat testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human beta-1,3-N-acetylglucosaminyltransferase 2/B3GNT2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3960) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for beta-1,3-N-acetylglucosaminyltransferase 2/B3GNT2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human/Rat beta-1,3-N-acetylglucosaminyl-transferase 2/B3GNT2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human brain tissue and rat testis tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2

B3-GNT (also B3GNT-2) is an 80 kDa (45 kDa without glycosylation) type II transmembrane  Golgi enzyme that belongs to the glycosyltransferase 31 family of enzymes. It catalyzes the transfer of N-acetyl glucosamine from UDP-GlcNAc onto Gal beta 3(GlcNAc beta 6)GalNAc-mucin. It functions in the elongation and branching of O-linked oligosaccharide chains of mucin glycoproteins. Over aa 29-397, human B3GNT2 shares 88% aa sequence identity with rat B3GNT2.

Long Name

UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyl-transferase 1

Alternate Names

B3Galt6, Beta3gnt1

Entrez Gene IDs

10678 (Human); 53625 (Mouse)

Gene Symbol

B3GNT2

UniProt

Q9NY97

Additional Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Products

Product Documents for Human/Rat beta-1,3-N-acetylglucosaminyl-transferase 2/B3GNT2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Rat beta-1,3-N-acetylglucosaminyl-transferase 2/B3GNT2 Antibody

For research use only

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