Detects human, mouse, and rat 14-3-3 eta in Western blots. In Western Blot less than 1% cross reactivity with other recombinant human 14-3-3 isoforms.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat 14-3-3 eta Antibody

Detection of Human/Mouse/ Rat 14‑3‑3 eta by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/ Mouse/Rat 14-3-3 eta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4420) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human 14-3-3 beta, epsilon, eta, gamma, sigma, theta, and zeta (2 ng/lane) were included. A specific band for 14-3-3 eta was detected at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat 14-3-3 eta Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and TS1 mouse helper T cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 14-3-3 eta

14-3-3 proteins are a highly conserved family of homo- and heterodimeric phosphoserine/threonine-binding proteins present in all eukaryotic cells. 14-3-3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs phosphorylated on serine or threonine. There are at least 7 distinct mammalian 14-3-3 genes: alpha/ beta, epsilon, eta, gamma, tau/ theta, sigma, and zeta/ delta.

Alternate Names

1433 eta, Protein AS1, YWHA1, YWHAH

Entrez Gene IDs

7533 (Human); 22629 (Mouse); 25576 (Rat)

Gene Symbol

YWHAH

UniProt

Q04917

Additional 14-3-3 eta Products

Product Documents for Human/Mouse/Rat 14-3-3 eta Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse/Rat 14-3-3 eta Antibody

For research use only

Citations
Reviews

Human/Mouse/Rat 14-3-3 eta Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4420

Key Product Details

Species Reactivity

Validated:

Cited:

Applications