Western Blot shows lysates of RL human non-Hodgkin's lymphoma B cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/ml of Goat Anti-Human/Mouse BCAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4857) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for BCAP at approximately 80 kDa and 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Human BCAP/PIK3AP1 by Western Blot

BCAP is hyperphosphorylated in B-cell, macrophages, and Expi293F cells.A, lysates from THP-1 and Ramos cells were dephosphorylated with lambda-phosphatase and immunoblotted for BCAP. B, His-Avi-tagged BCAP expressed in Expi293F cells was purified and dephosphorylated with lambda-phosphatase before immunostaining for tyrosine and serine phosphorylation. C, phosphorylation sites of BCAP expressed in Expi293F cells were determined by phosphopeptide mapping. BCAP was digested with trypsin, chymotrypsin, Asp-N, and Glu-C prior to MS. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31527084), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human BCAP/PIK3AP1 by Western Blot

BCAP is phosphorylated by BTK, LYN, and SYK. Purified BCAP, dephosphorylated BCAP ( lambda-BCAP), and myelin basic protein (MyBP) were phosphorylated with (A) tyrosine kinases SYK, LYN, BTK, TYK2, and ITK, or (B) serine kinases CSNK1A1, CSNK2A2, and GSK-3. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31527084), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Applications for Human/Mouse BCAP Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: RL human non-Hodgkin's lymphoma B cell line and Daudi human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BCAP

BCAP (B-cell adaptor for phosphoinositide 3-kinase (PI3K)) participates in linking the B cell antigen receptor (BCR) with the PI3K pathway. Tyrosine phosphorylation of BCAP by BCR-associated protein tyrosine kinases, such as Syk and Btk, generates binding sites for the p85 subunit of PI3K, resulting in activation of the PI3K pathway. Human and mouse BCAP contain 3 YXXM SH2 binding motifs for interaction with PI3K.

Long Name

B Cell Adaptor Protein

Alternate Names

Pik3ap1

Entrez Gene IDs

118788 (Human); 83490 (Mouse); 294048 (Rat)

Gene Symbol

PIK3AP1

UniProt

Q6ZUJ8

Additional BCAP Products

Product Documents for Human/Mouse BCAP Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse BCAP Antibody

For research use only

Citations
Reviews