Western blot shows lysates of Y-79 human retinoblastoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CRX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7085) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CRX at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

CRX in Y‑79 Human Cell Line.

CRX was detected in immersion fixed Y-79 human retinoblastoma cell line using Sheep Anti-Human CRX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7085) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human CRX Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed Y‑79 human retinoblastoma cell line

Western Blot

1 µg/mL
Sample: Y‑79 human retinoblastoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRX

CRX (Cone Rod homeoboX protein) is a 37-39 kDa member of the Otx family of paired-like homeodomain containing proteins. It is expressed in pinealocytes, cones, rods and rod bipolar cells, and promotes the transcription of photoreceptor cell proteins such as rhodopsin, IRBP and beta-phosphodiesterase. Although CRX may act alone, it typically binds noncovalently to both QRX and NRL, forming a transcriptional complex. Human CRX is 299 amino acids (aa) in length. It contains one DNA‑binding homeobox domain (aa 39-98). There is one isoform variant that shows a deletion of aa 146-149. Over aa 166-299, human CRX shares 97% aa identity with mouse CRX.

Long Name

Cone-rod Homeobox Protein

Alternate Names

CORD2, LCA7, OTX3

Entrez Gene IDs

1406 (Human); 12951 (Mouse); 60446 (Rat)

Gene Symbol

CRX

UniProt

O43186 (Human)

Additional CRX Products

Product Documents for Human CRX Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CRX Antibody

For research use only

Citations
Reviews

Human CRX Antibody

R&D Systems, part of Bio-Techne | Catalog # AF7085

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all CRX Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CRX Antibody

Detection of Human CRX by Western Blot.