Yoda 1

Name: Yoda 1
Brand: Tocris Bioscience
SKU: 5586
Rating: 5 (2 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 5586

(2)

Citations (179)

COA / SDS

Piezo1 channel activator

Key Product Details

Description:	Piezo1 channel activator
Chemical Name:	2-[5-[[(2,6-Dichlorophenyl)methyl]thio]-1,3,4-thiadiazol-2-yl]pyrazine
Purity:	≥98% (HPLC)
Molecular Weight:	355.27

View Full Technical Details for Yoda 1

Technical Data
Citations / Reviews (2)

Biological Activity for Yoda 1

Yoda 1 is a selective activator of mouse and human mechanosensitive channel piezo1. In HEK cells, Yoda 1 slows the inactivation phase of transient currents, sensitizes Piezo1 to activation by pressure, and partially activates channels in the absence of external pressure. In MDCK cells, Yoda 1 induces Ca²⁺-dependent chromatin hypercondensation and alters Ca²⁺-dependent myosin contractility, leading to nuclear shrinkage in cells. In red blood cells from sickle cell anemia (SCA) patients, Yoda 1 increases intracellular Ca²⁺ and phosphatidylserine exposure, which leads to KCa3.1 channel mediated Ca²⁺ influx and K⁺ and water efflux, causing shrinkage of the cells.

Compound Libraries for Yoda 1

Yoda 1 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for Yoda 1

M. Wt	355.27
Formula	C₁₃H₈Cl₂N₄S₂
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	448947-81-7
PubChem ID	2746822
InChi Key	BQNXBSYSQXSXPT-UHFFFAOYSA-N
Smiles	ClC1=C(CSC2=NN=C(C3=NC=CN=C3)S2)C(Cl)=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Yoda 1

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	7.11	20

Preparing Stock Solutions for Yoda 1

The following data is based on the product molecular weight 355.27.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	14.07 mL	70.37 mL	140.74 mL
1 mM	2.81 mL	14.07 mL	28.15 mL
2 mM	1.41 mL	7.04 mL	14.07 mL
10 mM	0.28 mL	1.41 mL	2.81 mL

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References for Yoda 1

References are publications that support the biological activity of the product.

Cahalan Piezo1 links mechanical forces to red blood cell volume. Elife. 2015 PMID: 26001274
Mikhailov Mechanosensitive meningeal nociception via Piezo channels: Implications for pulsatile pain in migraine? Neuropharmacology 2019 PMID: 30768945
Jetta Shear stress-induced nuclear shrinkage through activation of Piezo1 channels in epithelial cells. J.Cell Sci. 2019 PMID: 31076516
Syeda Chemical activation of the mechanotransduction channel Piezo1. ELife 2015 PMID: 26001275