Simple Plex Human CCL5/RANTES Cartridge

Name: Simple Plex Human CCL5/RANTES Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000231

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000231

Product Datasheet / COA

Simple Plex CCL5/RANTES assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), Platelet-poor EDTA Plasma (10 uL), Platelet-poor Heparin Plasma (10 uL)
Sensitivity:	0.296 pg/mL
Assay Range:	0.68 - 2,600 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)
Simple Plex Human CCL5/RANTES Cartridge Summary:	Simple Plex assay for the detection of human Regulated upon Activation, Normal T Cell Expressed and presumably Secreted (RANTES) in cell culture supernatant (CCS), serum, and plateletpoor plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human CCL5/RANTES Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human CCL5/RANTES Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human RANTES.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	28	24
Mean (pg/mL)	18.4	787	15.6	728
Standard Deviation	0.71	47.4	1.06	51.1
CV%	3.9	6	6.8	7

Linearity

Samples containing and/or spiked with high concentrations of human RANTES were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human CCL5/RANTES Cartridge

	Human RANTES Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL5/RANTES

CCL5/RANTES is a secreted beta-chemokine that plays a primary role in the inflammatory immune response through chemoattraction and activation of leukocytes that express CCR1, 3, 4, or 5. The two N-terminal residues of CCL5 can be removed by CD26/DPPIV, generating a protein that functions as a chemotaxis inhibitor. The deposition of CCL5 on activated vascular endothelial cells is crucial for monocyte adhesion to damaged vasculature. CCL5 is upregulated in breast cancer and promotes tumor progression through the attraction of proinflammatory macrophages in addition to its actions on tumor cells, stromal cells, and the vasculature. In humans, CCR5 binding to CCL5 inhibits the infectivity of R5 (M-tropic) but not X4 (T-tropic) strains of HIV-1.

Item	Value
Entrez Gene IDs	6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)
Alternate Names	CCL5, D17S136Enormally T-expressed, and presumably secreted, EoCP, Eosinophil chemotactic cytokine, RANTES, SIS-delta, SISd, Small-inducible cytokine A5, T cell-specific protein P228, T-cell specific protein p288, TCP228T-cell-specific protein RANTES, chemokine (C-C motif) ligand 5, small inducible cytokine A5 (RANTES), small inducible cytokine subfamily A (Cys-Cys), member 5

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human CCL5/RANTES Cartridge

Product Datasheets

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COA

Protocols & Manuals

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