Rat anti-Mouse IgM Heavy Chain Secondary Antibody [FITC]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43944
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry
Label
FITC (Excitation = 495 nm, Emission = 519 nm)
Antibody Source
Monoclonal Rat IgG2a Kappa
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Mouse IgM
Reactivity Notes
This antibody is reactive to Mouse.
Specificity
This secondary antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugate can be used as a detection secondary for mouse IgM.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2a Kappa
Scientific Data Images for Rat anti-Mouse IgM Heavy Chain Secondary Antibody [FITC]
Flow Cytometry: Rat anti-Mouse IgM Heavy Chain Secondary Antibody [FITC] [NBP1-43944] - Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) APC and 0.25 micrograms of Rat IgG2a k Isotype Control FITC (left) or 0.25 micrograms of Anti-Mouse IgM FITC (right). Total viable cells were used for analysis.
Applications for Rat anti-Mouse IgM Heavy Chain Secondary Antibody [FITC]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) with 0.1% gelatin
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: IgM Heavy Chain
Alternate Names
Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain
Gene Symbol
IGHM
Additional IgM Heavy Chain Products
Product Documents for Rat anti-Mouse IgM Heavy Chain Secondary Antibody [FITC]
Product Specific Notices for Rat anti-Mouse IgM Heavy Chain Secondary Antibody [FITC]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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