Key Product Details

Species Reactivity

Rat

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IgG Fc Secondary Antibodies »

Product Specifications

Immunogen

Rat IgG F(c) fragment

Specificity

No reaction was observed against Rat IgG F(ab).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum

Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Goat anti-Rat IgG Fc Secondary Antibody

Western Blot: Goat anti-Rat IgG Fc Secondary Antibody [NBP2-62582]

Western Blot: Goat anti-Rat IgG Fc Secondary Antibody [NBP2-62582]

Western Blot: Goat anti-Rat IgG Fc Secondary Antibody [NBP2-62582] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rat IgG F(c) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher. Image from the Texas Red version of this antibody.
Goat anti-Rat IgG Fc Secondary Antibody

Goat anti-Rat IgG Fc Secondary Antibody

Western blot of Goat Anti-Rat IgG F(c) secondary antibody. Lane 1: Rat Fc

Applications for Goat anti-Rat IgG Fc Secondary Antibody

Application
Recommended Usage

ELISA

1:20000 - 1:100000

Immunohistochemistry

1:1000 - 1:5000

Western Blot

1:2000 - 1:10000
Application Notes
This product has been tested by ELISA, dot blot, and western blot and is suitable for immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Product Documents for Goat anti-Rat IgG Fc Secondary Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Goat anti-Rat IgG Fc Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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