Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Pre-adsorbed

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IgG F(ab')2 Secondary Antibodies »

Product Specifications

Immunogen

Rabbit anti-Human IgG F(ab)2 Secondary Antibody (Pre-adsorbed)was produced in rabbit by repeated immunization with human IgG F(ab')2 fragment.

Specificity

This antibody was pre-adsorbed against Mouse Serum Proteins. No reaction was observed against Human IgG F(c) or Mouse Serum Proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG, Human IgG F(ab')2 and Human Serum

Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Rabbit anti-Human IgG F(ab')2 Secondary Antibody (Pre-adsorbed)

ELISA: Rabbit anti-Human IgG F(ab')2 Secondary Antibody (Pre-adsorbed) [NBP1-72785]

ELISA: Rabbit anti-Human IgG F(ab')2 Secondary Antibody (Pre-adsorbed) [NBP1-72785]

ELISA: Rabbit anti-Human IgG F(ab')2 Secondary Antibody (Pre-adsorbed) [NBP1-72785] - ELISA Results of Rabbit anti-Human IgG F(ab')2 Secondary antibody (Pre-adsorbed) (Min X Mouse Serum Proteins) tested against purified Human IgG F(ab')2 Mx Ms. Each well was coated in duplicate with 1.0 ug of Human IgG F(ab')2 [Red Line], Human IgG [Green Line], and Mouse IgG [Blue Line]. The working dilution of Human IgG F(ab')2 MX is 1:56300. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer, Goat Anti-Rabbit HRP conjugated, and TMB substrate.

Applications for Rabbit anti-Human IgG F(ab')2 Secondary Antibody (Pre-adsorbed)

Application
Recommended Usage

ELISA

1:350000

Immunohistochemistry

1:1000 - 1:5000

Western Blot

1:3000 - 1:15000
Application Notes
This product has been tested by ELISA and dot blot and is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

Pre-adsorbed

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Product Documents for Rabbit anti-Human IgG F(ab')2 Secondary Antibody (Pre-adsorbed)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Rabbit anti-Human IgG F(ab')2 Secondary Antibody (Pre-adsorbed)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.