Goat anti-Bovine IgG F(ab')2 Secondary Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72717
Key Product Details
Species Reactivity
Bovine
Applications
Dot Blot, ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Bovine IgG F(ab')2 fragment
Specificity
No reaction was observed against Bovine IgG F(c).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Bovine IgG, Bovine IgG F(ab')2 and Bovine Serum
Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Scientific Data Images for Goat anti-Bovine IgG F(ab')2 Secondary Antibody
Dot Blot: Goat anti-Bovine IgG F(ab')2 Secondary Antibody [NBP1-72717] - Dot Blot of Goat anti-Bovine IgG Fab2 Antibody Fluorescein Conjugated. Antigen: Bovine IgG. Load: Lane 1 - 100 ng Lane 2 - 33.3 ng Lane 3 - 11.1 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng. Primary antibody: n/a. Secondary antibody: Goat anti-Bovine IgG Fab2 Antibody Fluorescein Conjugated at 1:1,000 for 1 HR at RT. Blocked with blocking buffer for 60 min at RT. Image from the FITC version of this antibody.
Applications for Goat anti-Bovine IgG F(ab')2 Secondary Antibody
Application
Recommended Usage
ELISA
1:20000 - 1:100000
Immunohistochemistry
1:1000 - 1:5000
Western Blot
1:2000 - 1:10000
Application Notes
This product is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IgG F(ab')2
Additional IgG F(ab')2 Products
Product Documents for Goat anti-Bovine IgG F(ab')2 Secondary Antibody
Product Specific Notices for Goat anti-Bovine IgG F(ab')2 Secondary Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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