Recombinant SARS-CoV-2 B.1.621 Spike GCN4-IZ His Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10899-CV

Mu Variant
R&D Systems, part of Bio-Techne
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10899-CV-100
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Key Product Details

Source

HEK293

Accession #

YP_009724390.1

Conjugate

Unconjugated

Applications

Bioactivity

View all Spike Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived sars-cov-2 Spike protein
SARS-CoV-2 B.1.621 Spike
(Val16-Lys1211)(Thr95Ile, Tyr144Thr, Tyr145Ser, ins145Asn, Arg346Lys, Glu484Lys, Asn501Tyr, Asp614Gly, Pro681His, Asp950Asn) (Arg682Ser, Arg685Ser, Lys986Pro, Val987Pro)
Accession # YP_009724390.1		 GCN4-IZ 6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val 16

Predicted Molecular Mass

138 kDa

SDS-PAGE

142-170 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA with Recombinant Human ACE-2 His-tag (Catalog # 933-ZN).

Scientific Data Images for Recombinant SARS-CoV-2 B.1.621 Spike GCN4-IZ His Protein, CF

Recombinant SARS-CoV-2 B.1.621 Spike GCN4-IZ His-tag Protein Binding Activity.

Recombinant SARS-CoV-2 B.1.621 Spike GCN4-IZ His-tag (Catalog # 10899-CV) binds Recombinant Human ACE-2 His-tag (Catalog # 933-ZN) in afunctional ELISA.

Recombinant SARS-CoV-2 B.1.621 Spike GCN4-IZ His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant SARS-CoV-2 AY.1/AY.2 Spike RBD His-tag Protein (Catalog # 10899-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 142-170 kDa.

Formulation, Preparation and Storage

10899-CV
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Spike

SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that also include MERS and SARS-CoV-1. Coronaviruses are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M) and Nucleocapsid protein (N) (1). The SARS-CoV-2 S protein is a glycoprotein that mediates membrane fusion and viral entry. The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 (2). In SARS-CoV-2, as with most coronaviruses, proteolytic cleavage of the S protein into S1 and S2 subunits is required for activation. The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion (3-5). A metallopeptidase, angiotensin-converting enzyme 2 (ACE2), has been identified as a functional receptor for SARS-CoV-2 through interaction with a receptor binding domain (RBD) located at the C-terminus of S1 subunit (6,7). The S protein of SARS-CoV-2 shares 75% and 29% amino acid (aa) sequence identity with the S protein of SARS-CoV-1 and MERS, respectively. A SARS-CoV-2 variant (B.1.621) carrying the aa substitution Thr95Ile, Tyr144Thr, Try145Ser, ins145Asn, Arg346Lys, Glu484Lys, Asn501Tyr, Asp614Gly, Pro681His, Asp950Asn in the Spike protein was originally identified in samples from Colombia and spreaded rapidly to US and Europe (8). Whether these mutations in the spike protein would cause more severe symptom or decrease the efficacy of vaccine-induced immunity is still under investigation.

References

  1. Wu, F. et al. (2020) Nature 579:265.
  2. Tortorici, M.A. and D. Veesler (2019) Adv. Virus Res. 105:93.
  3. Bosch, B.J. et al. (2003) J. Virol. 77:8801.
  4. Belouzard, S. et al. (2009) Proc. Natl. Acad. Sci. 106:5871.
  5. Millet, J.K. and G.R. Whittaker (2015) Virus Res. 202:120.
  6. Li, W. et al. (2003) Nature 426:450.
  7. Wong, S.K. et al. (2004) J. Biol. Chem. 279:3197.
  8. Laiton-Donato, K. et al. (2021) MedRxiv https://www.medrxiv.org/content/10.1101/2021.05.08.21256619v2.full-text

Long Name

Spike Protein

Alternate Names

S Protein

Entrez Gene IDs

918758 (HCoV-229E); 2943499 (HCoV-NL63); 39105218 (HCoV-OC43); 37616432 (MERS-CoV); 1489668 (SARS-CoV); 43740568 (SARS-CoV-2)

Gene Symbol

S

UniProt

YP_009724390.1

Additional Spike Products

Product Documents for Recombinant SARS-CoV-2 B.1.621 Spike GCN4-IZ His Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant SARS-CoV-2 B.1.621 Spike GCN4-IZ His Protein, CF

For research use only

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