Recombinant Mouse Skint2/B7S3 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10279-B7

R&D Systems, part of Bio-Techne
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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse Skint2/B7S3 Fc Chimera Protein (10279-B7)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession #

NP_001272892.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Skint2/B7S3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Skint2/B7S3 protein
Mouse Skint2/B7S3
(Ser22-Lys239)
Accession # NP_001272892.1		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser22

Predicted Molecular Mass

52 kDa

SDS-PAGE

48-64 kDa, under reducing conditions

Activity

Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3.
The ED50 for this effect is 0.5-5.0 µg/mL.

Scientific Data Images for Recombinant Mouse Skint2/B7S3 Fc Chimera Protein, CF

Recombinant Mouse Skint2/B7S3 Fc Chimera Protein Bioactivity

Recombinant Mouse Skint2/B7S3 Fc Chimera Protein Bioactivity

Recombinant Mouse Skint2/B7S3 Fc Chimera (Catalog # 10279-B7) inhibits IL-2 secretion by mouse T cells in the presence of Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841). The ED50 for this effect is 0.5-5.0 µg/mL.
Recombinant Mouse Skint2/B7S3 Fc Chimera Protein SDS-PAGE

Recombinant Mouse Skint2/B7S3 Fc Chimera Protein SDS-PAGE

1 μg/lane of Recombinant Mouse Skint2/B7S3 Fc Chimera (Catalog # 10279-B7) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 48-64 kDa and 95-130 kDa, respectively.

Formulation, Preparation and Storage

10279-B7
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Skint2/B7S3

B7S3 (B7 superfamily member 3) is a member of the B7 superfamily which play a major role in maintaining the appropriate threshold of T cell activation. B7S3 has two isoforms expressed in both lymphoid and non-lymphoid tissues (1). The mature extracellular domain (ECD) of mouse B7S3 contains an IgV-like domain, and a half IgC-like domain (1). Within the ECD, mouse B7S3 shares 89% amino acid sequence identity with rat B7S3. A putative receptor for B7S3 is constitutively expressed on APCs and induced on T cells upon activation. Mouse B7S3 mFc chimera inhibited CD4 and CD8 T cell proliferation and IL-2 production, as well as effector cytokine production. Interestingly, the B7S3 genes in mouse and human have distinct structural features (1).

References

  1. Yang, Y. et al. (2007) J. Immunol. 178:3661.

Long Name

Selection and Upkeep of Intraepithelial T Cells 2

Alternate Names

B7S3

Entrez Gene IDs

329919 (Mouse)

Gene Symbol

SKINT2

UniProt

NP_001272892.1

Additional Skint2/B7S3 Products

Product Documents for Recombinant Mouse Skint2/B7S3 Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Mouse Skint2/B7S3 Fc Chimera Protein, CF

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