Recombinant Mouse NTB-A/SLAMF6 Fc Chimera Protein, CF
Catalog # 3986-NT | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Accession #
Source
Structure / Form
Conjugate
Applications
Product Specifications
Source
Mouse NTB-A (Glu31 - Asn239) Accession # Q9ET39 |
IEGRMD | Mouse IgG2a (Glu98 - Lys330) |
N-terminus | C-terminus |
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
Formulation, Preparation and Storage
3986-NT
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
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Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: NTB-A/SLAMF6
NTB-A, also known as Ly108 and SLAMF6, is a 60 kDa type I transmembrane glycoprotein that belongs to the SLAM subgroup of the CD2 family (1). Mature mouse NTB-A consists of a 209 amino acid (aa) ECD with one Ig-like V-type and one Ig-like C2-type domain, a 23 aa transmembrane segment, and an 89 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs ITSMs (2). Within the ECD, mouse NTB-A shares 48% and 70% aa sequence identity with human and rat NTB-A, respectively. The ECD of mouse NTB-A shares 20% - 34% aa sequence identity with comparable regions of mouse 2B4, BLAME, CD2F-10, CD84, CD229, CRACC, and SLAM. An alternatively spliced isoform diverges after the second ITSM (2). NTB-A is expressed on the surface of NK, T, and B lymphocytes as well as eosinophils (3 - 5). It interacts homophilically through weak associations between the Ig-V type domains (5 - 7). NTB-A functions as an activating coreceptor on NK and T cells (3, 5, 6, 8). Tyrosine phosphorylation in the membrane proximal ITSM enables specific association with EAT-2, an interaction that is required for NTB-A mediated cytotoxicity of NK cells (9). Phosphorylation-dependent NTB-A association with SAP is required for full production of NK cell IFN-gamma (5, 9). This interaction is independent of EAT-2 binding and appears to involve the membrane distal ITSM (5, 9). NTB-A deficient mice show weakened Th2 responses and elevated levels of neutrophil-derived inflammatory mediators (10). On B cells, NTB-A modulates immunoglobulin class switching and the balance between tolerance and autoimmunity (5, 11). The isoform with the divergent C-terminal tail is overexpressed in B cells from lupus-prone mice (11).
References
- Veillette, A. (2006) Immunol. Rev. 214:22.
- Peck, S.R. and H.E. Ruley (2000) Immunogenetics 52:63.
- Bottino, C. et al. (2001) J. Exp. Med. 194:235.
- Munitz, A. et al. (2005) J. Immunol. 174:110.
- Valdez, P.A. et al. (2004) J. Biol. Chem. 279:18662.
- Flaig, R.M. et al. (2004) J. Immunol. 172:6524.
- Cao, E. et al. (2006) Immunity 25:559.
- Stark, S. and C. Watzl (2006) Int. Immunol. 18:241.
- Eissmann, P. and C. Watzl (2006) J. Immunol. 177:3170.
- Howie, D. et al. (2005) J. Immunol. 174:5931.
- Kumar, K.R. et al. (2006) Science 312:1665.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Product Documents for Recombinant Mouse NTB-A/SLAMF6 Fc Chimera Protein, CF
Product Specific Notices for Recombinant Mouse NTB-A/SLAMF6 Fc Chimera Protein, CF
For research use only
Citations for Recombinant Mouse NTB-A/SLAMF6 Fc Chimera Protein, CF (2)
Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse NTB-A/SLAMF6 Fc Chimera Protein, CF include:
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Species: Mouse
Sample Types: Whole Cells
Applications: BioassayY Baglaenko et al. (2017-04-03), Invariant NKT Cell Activation Is Potentiated by Homotypic trans-Ly108 Interactions J. Immunol., 2017-00(0).
PMID: 28373584 -
Species: Mouse
Sample Types: Whole Cells
Applications: NeutralizationDutta et al. (2013-01-25), A role for Ly108 in the induction of promyelocytic zinc finger transcription factor in developing thymocytes. J Immunol, 2013-0190(5):2121-8.
PMID: 23355739
There are no citations that match your criteria.
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