Recombinant Influenza A Virus H1N1 Hemagglutinin Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 11202-HA
His-tag (Sf21-Expressed)
Key Product Details
- R&D Systems Sf 21 (baculovirus)-derived Recombinant Influenza A Virus H1N1 Hemagglutinin Protein (11202-HA)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived influenza a virus h1n1 Hemagglutinin protein
Asp18-Gln529, with a C-terminal 6-His tag
Asp18-Gln529, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Asp18
Predicted Molecular Mass
58 kDa
SDS-PAGE
55-70 kDa, under reducing conditions.
Activity
Measured by its binding ability in a functional ELISA.
When Recombinant Human Galectin-1 (Catalog # 1152-GA) immobilized at 10.00 μg/mL, 100 μL/well, the concentration of Recombinant Influenza A Virus H1N1 Hemagglutinin His-tag (Catalog # 11202-HA) that produces 50% of the optimal binding response is 0.500-5.00 μg/mL.
When Recombinant Human Galectin-1 (Catalog # 1152-GA) immobilized at 10.00 μg/mL, 100 μL/well, the concentration of Recombinant Influenza A Virus H1N1 Hemagglutinin His-tag (Catalog # 11202-HA) that produces 50% of the optimal binding response is 0.500-5.00 μg/mL.
Scientific Data Images for Recombinant Influenza A Virus H1N1 Hemagglutinin Protein, CF
Recombinant Influenza A Virus H1N1 Hemagglutinin His-tag Protein Binding Activity.
Measured by its binding ability in a functional ELISA. When Recombinant Human Galectin-1 (1152-GA) immobilized at 10.00 μg/mL, 100 μL/well, the concentration of Recombinant Influenza A Virus H1N1 Hemagglutinin His-tag Protein (Catalog # 11202-HA) that produces 50% of the binding response is 0.500-5.00 μg/mL.
Recombinant Influenza A Virus H1N1 Hemagglutinin Protein SDS-PAGE.
2 μg/lane of Recombinant Influenza A Virus H1N1 Hemagglutinin Protein (Catalog # 11202-HA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-70 kDa.Formulation, Preparation and Storage
11202-HA
| Formulation | Supplied as a 0.2 μm filtered solution in PBS with Trehalose. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Hemagglutinin
References
- Michal Lazniewski, M. et al. (2018) Briefings in Functional Genomics 17:415.
- Sriwilaijaroen, N. and Suzuki, Y. (2012) Proc Jpn Acad Ser B Phys Biol Sci. 88:226.
- Chang, Y.J. et al. (2021) Sci Rep 11:8637.
- Short, K. et al. (2018) Front. Cell. Infect. Microbiol. 8:343.
Long Name
Hemagglutinin
Alternate Names
HA
Entrez Gene IDs
23308115 (Influenza A Virus H1N1)
Gene Symbol
HA
UniProt
Additional Hemagglutinin Products
Product Documents for Recombinant Influenza A Virus H1N1 Hemagglutinin Protein, CF
Product Specific Notices for Recombinant Influenza A Virus H1N1 Hemagglutinin Protein, CF
For research use only
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