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Recombinant Influenza A Virus H1N1 Hemagglutinin Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10973-HA

His-tag (HEK293-Expressed)
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10973-HA-100

Key Product Details

Source

HEK293

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived influenza a virus h1n1 Hemagglutinin protein
Asp18-Gln529, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

N-terminal Sequence Analysis

Asp18

Predicted Molecular Mass

58 kDa

SDS-PAGE

68-83 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Galectin-1 (Catalog # 1152-GA) immobilized at 10.00 μg/mL, 100 μL/well, the concentration of Recombinant Influenza A Virus H1N1 HA Tag His-tag (Catalog # 10973-HA) that produces 50% of the optimal binding response is 0.8-8.0 μg/mL.

Scientific Data Images for Recombinant Influenza A Virus H1N1 Hemagglutinin Protein, CF

Recombinant Influenza A Virus H1N1 Hemagglutinin His-tag Protein Binding Activity.

When Recombinant Human Galectin-1 (1152-GA) immobilized at 10.00 μg/mL, 100 μL/well, the concentration of Recombinant Influenza A Virus H1N1 Hemagglutinin His-tag Protein (Catalog # 10973-HA) that produces 50% of the optimal binding response is 0.800-8.00 μg/mL.

Recombinant Influenza A Virus H1N1 Hemagglutinin His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Influenza A Virus H1N1 Hemagglutinin His-tag Protein (Catalog # 10973-HA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 68-83 kDa.

Formulation, Preparation and Storage

10973-HA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hemagglutinin

Hemagglutinin (HA) and neuraminidase (NA) are major membrane glycoproteins found on the surface of influenza virus. The two proteins are essential for the infectious cycle of the influenza virus. During initial infection, HA binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. HA exists as a homotrimer, with each monomer composed of a globular HA1 domain and the stalk-like HA2 domain. HA has evolved to recognize long alpha2-6 sialylated glycans for efficient transmission in human population.  Although the HA sequence patterns of the 1918 pandemic and 2009 pandemic viruses are similar, they were caused by different H1N1 subtype viruses. The H1N1 1918 HA shares 86% amino acid sequence identity with the H1N1 2009 HA. Inhibiting HA-mediated membrane fusion is a target for influenza therapies. The HA section in the 1918 virus is being currently used to investigate how the 1918 pandemic spread and how a modern pandemic influenza virus would play out in the future.

References

  1. Michal Lazniewski, M. et al. (2018) Briefings in Functional Genomics 17:415.
  2. Sriwilaijaroen, N. and Suzuki, Y. (2012) Proc Jpn Acad Ser B Phys Biol Sci. 88:226.
  3. Chang, Y.J. et al. (2021) Sci Rep 11:8637.
  4. Short, K. et al. (2018) Front. Cell. Infect. Microbiol. 8:343.

Long Name

Hemagglutinin

Alternate Names

HA

Entrez Gene IDs

23308115 (Influenza A Virus H1N1)

Gene Symbol

HA

UniProt

Additional Hemagglutinin Products

Product Documents for Recombinant Influenza A Virus H1N1 Hemagglutinin Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Influenza A Virus H1N1 Hemagglutinin Protein, CF

For research use only

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