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Recombinant Human LRRN4 His-tag Protein, CF

Catalog # 10227-LR | R&D Systems, Inc. a Bio-Techne Brand
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10227-LR-050

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human LRRN4 protein
Asp19-Ser679, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Predicted Molecular Mass

71 kDa

SDS-PAGE

106-123 kDa, under reducing conditions

Activity

Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Human LRRN4 His-tag (Catalog # 10227-LR), immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly inhibit neurite outgrowth.

Scientific Data Images for Recombinant Human LRRN4 His-tag Protein, CF

Recombinant Human LRRN4 His-tag Protein SDS-PAGE

Recombinant Human LRRN4 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human LRRN4 His-tag (Catalog # 10227-LR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 106-123 kDa.

Formulation, Preparation and Storage

10227-LR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRRN4

LRRN4 (Leucine-rich repeat neuronal protein 4) is a type I transmembrane protein that is a member of the LRRN family. It is expressed in lung, heart, ovary, and neuronal tissues (1-3). Mature human LRRN4 is composed of a 661 amino acid (aa) extracellular domain (ECD) that includes 10 LRRs and a fibronectin type III-like domain, a 21 aa transmembrane segment, and a 40 aa cytoplasmic domain. Within the ECD, human LRRN4 shares 66% and 64% aa sequence identity with mouse and rat LRRN4, respectively. LRRN-4-deficient mice show defects in the memory retention, suggesting this protein may be involved in memory retention (4). In addition, LRRN4 is found in about 8% dorsal root ganglion (DEG) neurons (2). These neurons are small-sized neurons that function as nociceptors. LRRN4 expression was decreased in the DRG by sciatic axotomy suggesting that LRRN4 might function as a synaptic adhesion molecule to maintain nociceptive circuits (2). LRRN4 is also expressed in primary mesothelial cells and may be developed as a marker for detection of mesothelioma antigens (5).

References

  1. Bando, T. et al. (2005) Mol. Cell. Biol. 25:4166.
  2. Bando, T. et al. (2012) Neurosci. Lett. 531:24.
  3. Bando, T. et al. (2013) Neurosci. Lett. 548:73.
  4. Bando, T. et al. (1995) Mol. Cellular. Biol. 25:4166.
  5. Kanamori-Katayama, M. (2011) PloS. One. 6:e24391.

Long Name

Leucine-rich Repeat Neuronal 4

Alternate Names

C20orf75, dJ1056H1.1, NLRR4

Entrez Gene IDs

164312 (Human); 320974 (Mouse); 311443 (Rat)

Gene Symbol

LRRN4

UniProt

Product Documents for Recombinant Human LRRN4 His-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human LRRN4 His-tag Protein, CF

For research use only

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