Recombinant Human Kallikrein 2 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11691-SE

CHO expressed
R&D Systems, part of Bio-Techne
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11691-SE-020
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Key Product Details

  • R&D Systems CHO-derived Recombinant Human Kallikrein 2 His-tag Protein (11691-SE)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession #

P20151.1

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Kallikrein 2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Kallikrein 2 protein
Ile25-Pro261, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile25

Predicted Molecular Mass

28 kDa

SDS-PAGE

22-32 kDa, under non-reducing conditions

Activity

Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC).
The specific activity is >250 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Human Kallikrein 2 His-tag Protein, CF

Recombinant Human Kallikrein 2 His-tag Protein Enzyme Activity.

Recombinant Human Kallikrein 2 His-tag (Catalog # 11691-SE) is measured by its ability to cleave the fluorogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC).

Formulation, Preparation and Storage

11691-SE
Formulation Supplied as a 0.2 μm filtered solution in Sodium Citrate and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Kallikrein 2

Recombinant human Kallikrein 2 (KLK2) is also known as glandular kallikrein or the prostate-specific glandular kallikrein as it is structurally related to KLK3, the prostate-specific antigen (PSA). KLK2 is a secreted serine protease member of the tissue kallikrein subfamily family of the peptidase S1 family (1). It is highly expressed in the human prostate gland (2) and synthesized as a pre-proenzyme like other kallikriens. Removal of the propeptide occurs through cleavage by trypsin-like activity to activate the protein (1).  KLK2 also contains a catalytic domain with an extended 99 or kallikrein loop (3) and glycan that regulate its activity (4). KLK2 is highly specific for cleavage after arginine residues and is able to autoactivate and play a role in activation of KLK cascades (5). In addition, KLK2 has been reported to activate other key proteins including the urokinase-type plasminogen activator (6), IGFBPs (7), and IL10 (8). Its activity is highly regulated by activation cascades, endogenous inhibitors such as serpins protein C inhibitor, antichymotrypsin, and plasminogen activator inhibitor 1 (9-11), pH, and metal dependency (1). It is heavily implicated to play a role in prostate cancer and is attractive as a biomarker and therapeutic target (12-15). 

References

  1. Prassas, I. et al. (2015) Nat. Rev. 14:183. 
  2. Chapdelaine, P. et al. (1988) FEBS Lett. 236:205.
  3. Skala, W. et al. (2014) J. Biol. Chem. 289:34267.
  4. Guo, S. et al. (2016) J. Biol. Chem. 291:593. 
  5. Yoon, H. et al. (2007) J. Biol. Chem. 282:31852.
  6. Frenette, G. et al. (1997) Int. J. Cancer 71:897.
  7. Emami, N. and E.P. Diamandis (2007) Mol. Oncol. 1:269.
  8. Oliveira, J.R. et al. (2024) Biochemistry 63:2023.
  9. Deperthes, D. et al. (1995) Biochim. Biophys. Acta 1245:311.
  10. Grauer, L.S. et al. (1998) J. Androl. 19:407.
  11. Mikolajczyk S.D. et al. (1999) Cancer Res. 59:3927.
  12. Sardana, G. et al. (2008) Clin. Chem. 54:1951.
  13. Shang, Z. et al. (2014) Tumor Biol. 35:1881.
  14. Bonk, S. et al. (2020) Prostate 80:1097.
  15. Zhang, J. and J.S. Chadha (2024) Cancers 16:3098.

Alternate Names

Klk1c2, KLK2, KLK2A2, Ton

Entrez Gene IDs

3817 (Human); 24841 (Rat); 102139474 (Cynomolgus Monkey)

Gene Symbol

KLK2

UniProt

P20151.1

Additional Kallikrein 2 Products

Product Documents for Recombinant Human Kallikrein 2 His-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Kallikrein 2 His-tag Protein, CF

For research use only

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