Recombinant Human IgE Fc His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11647-IE

R&D Systems, part of Bio-Techne
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11647-IE-100
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Key Product Details

  • R&D Systems CHO-derived Recombinant Human IgE Fc His-tag Protein (11647-IE)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession #

P01854-1

Structure / Form

Disulfide linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all IgE Fc Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human IgE Fc protein
Ser106-Lys428, with an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

37 kDa

SDS-PAGE

40-56 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human IgE Fc His-tag binds to Recombinant Human Fc epsilon RI alpha Fc Chimera Protein (Catalog # 11561-FC) with an ED50 of 0.100-1.00 ng/mL.

Scientific Data Images for Recombinant Human IgE Fc His-tag Protein, CF

Recombinant Human IgE Fc His-tag Protein Binding Activity.

Measured by its binding ability in a functional ELISA. Recombinant Human IgE Fc His-tag Protein (Catalog # 11647-IE) binds to Recombinant Human Fc epsilon RI alpha Fc Chimera Protein ( 11561-FC) with an ED50 of 0.100-1.00 ng/mL.

Recombinant Human IgE Fc His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human IgE Fc His-tag Protein (Catalog # 11647-IE) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-56 kDa, under reducing conditions.

Formulation, Preparation and Storage

11647-IE
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgE Fc

IgE is an immunoglobulin that plays a critical role in the immune response to parasitic infections and in the development of allergic reactions. Like other antibody classes, IgE has the same molecular architecture that includes two heavy chains and two light chains, but its heavy chain contains an additional domain compared to IgG heavy chain. IgE binds to its receptor, Fc epsilonRI, on the surface of mast cells and basophils, triggering the release of histamine and other mediators that cause the symptoms of allergic reactions (1, 2). The recombinant human IgE-Fc protein is a valuable tool for researchers studying the mechanisms of allergic reactions and the development of new therapies for allergic diseases. It can be used in a wide range of applications, including ELISA assays, Western blotting, functional assays to study the binding of IgE to its receptor, Fc epsilonRI, and the downstream signaling pathways that are activated upon binding (1, 2, 3). In addition to its research applications, the recombinant human IgE-Fc protein has potential therapeutic applications in the treatment of allergic diseases. Therapies that target IgE have been shown to be effective in the treatment of allergic diseases such as asthma and atopic dermatitis (4).

References

  1. Gould, H. J. and B. J. Sutton (2008) Nat. Rev. Immunol. 8:205.
  2. Karagiannis, S. N. et al. (2003) Eur. J. Immunol. 33:1030.
  3. Maurer, D. et al. (1996) J. Immunol. 157:607.
  4. Galli, S. J. and T. Mindy. (2012) Nat Med. 18:693.

Long Name

Immunoglobulin E Fc

Alternate Names

constant region of heavy chain of IgE, immunoglobulin epsilon, immunoglobulin heavy constant epsilon

Entrez Gene IDs

3497 (Human)

Gene Symbol

IGHE

UniProt

P01854-1

Additional IgE Fc Products

Product Documents for Recombinant Human IgE Fc His-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human IgE Fc His-tag Protein, CF

For research use only

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