Recombinant Human Fc gamma RIIIB/CD16b Protein, CF
Catalog # 1597-FC/CF | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Product Specifications
Source
Thr20-Gln208, with a C-terminal 10-His tag
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
Scientific Data Images for Recombinant Human Fc gamma RIIIB/CD16b Protein, CF
Recombinant Human Fc gamma RIIIB/CD16b Protein Binding Activity
Recombinant Human Fc gamma RIIIB/CD16b (Catalog # 1597-FC/CF) binds human IgG with an estimated KdRecombinant Human Fc gamma RIIIB/CD16b Protein SDS-PAGE
1 μg/lane of Recombinant Human Fc gamma RIIIB/CD16b was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 43-60 kDa.Formulation, Preparation and Storage
1597-FC/CF
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
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Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Fc gamma RIIIB/CD16b
Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily. Based on their genetic organization and molecular structure, three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized (1 - 3). These receptors function in the activation or inhibition of immune responses. The activating-type receptor either has, or associates non-covalently with an accessory subunit (FcR gamma or zeta chain) that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. In contrast, the inhibitory receptor (Fc gamma RIIB) has a built-in immunoreceptor tyrosine-based inhibitory motif (ITIM) in its own cytoplasmic domain. Fc gamma RI is a high-affinity receptor that binds monomeric IgG. Both Fc gamma RII and RIII are low-affinity receptors that bind IgG in the form of immune complexes. Two genes for human Fc gamma RIII, A and B, encoding a transmembrane receptor and a glycosylphosphatidylinositol (GPI) anchored protein, respectively, have been identified. Three allelic variants of Fc gamma RIIIB, NA-1, NA-2, and SH, exist. A soluble form of Fc gamma RIIIB corresponding to the extracellular region of the receptor is produced by proteolytic cleavage and circulates in plasma and other body fluids. The extracellular domains of Fc gamma RIIIA and B share 97% amino acid sequence homology. Whereas Fc gamma RIIIA is expressed on most effector cells of the immune system including macrophage, monocyte, NK cells, mast cells, eosinophils, dendritic cells and Langerhans cells, Fc gamma RIIIB is selectively expressed in neutrophils and eosinophils. Signaling through Fc gamma RIIIA results in oxidative burst, cytokine release and phagocytosis by macrophages, antibody-dependent cellular cytotoxicity by natural killer cells and degranulation of mast cells. By contrast, Fc gamma RIIIB is a decoy receptor that binds IgG complexes without triggering activation. Soluble Fc gamma RIIIB has a regulatory role in inflammatory processes (4). It interacts with complement receptors CR3 and CR4 on monocytes to induce the production of pro-inflammatory cytokines.
References
- van de Winkel, J, and P. Capes (1993) Immunol. Today 14:215.
- Ravetch, J.V. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
- Takai, T. (2002) Nature Rev. Immunol. 2:580.
- Gauchat, G.J. et al. (1996) J. Immunol. 157:1184.
Long Name
Alternate Names
Gene Symbol
UniProt
Product Documents for Recombinant Human Fc gamma RIIIB/CD16b Protein, CF
Product Specific Notices for Recombinant Human Fc gamma RIIIB/CD16b Protein, CF
For research use only
Citations for Recombinant Human Fc gamma RIIIB/CD16b Protein, CF (7)
Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Fc gamma RIIIB/CD16b Protein, CF include:
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Species: Human
Sample Types: Recombinant Protein
Applications: ELISA CaptureY Kitanaga et al. (2021-11-12), Discovery of a novel Igbeta and FcgammaRIIB cross-linking antibody, ASP2713, and its potential application in the treatment of systemic lupus erythematosus International immunopharmacology, 2021-1101(0):108343.
PMID: 34781122 -
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA (Capture)PA Blundell et al. (2019-01-25), Insertion of N-Terminal Hinge Glycosylation Enhances Interactions of the Fc Region of Human IgG1 Monomers with Glycan-Dependent Receptors and Blocks Hemagglutination by the Influenza Virus J. Immunol., 2019-00(0).
PMID: 30683699 -
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPRN Seo et al. (2018-04-20), Analytical and functional similarity of Amgen biosimilar ABP 215 to bevacizumab MAbs, 2018-00(0):1-58.
PMID: 29553864 -
Species: Human
Sample Types: Whole Tissue
Applications: BioassayXie J et al. (2014-03-26), Engineering of a novel anti-CD40L domain antibody for treatment of autoimmune diseases. J Immunol, 2014-0192(9):4083-92.
PMID: 24670803 -
Sample Types: Chip
Applications: Surface Plasmon ResonanceDerer S et al. (2013-12-11), Increasing FcgammaRIIa affinity of an FcgammaRIII-optimized anti-EGFR antibody restores neutrophil-mediated cytotoxicity. MAbs, 2013-16(2):409-21.
PMID: 24492248 -
Species: Human
Sample Types: DNA
Applications: EMSABoltz A et al. (2011-04-29), Bi-specific Aptamers Mediating Tumor Cell Lysis. J. Biol. Chem., 2011-0286(24):21896-905.
PMID: 21531729 -
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA (Capture)Bergin DA et al. (2010-11-08), Alpha-1 Antitrypsin regulates human neutrophil chemotaxis induced by soluble immune complexes and IL-8. J. Clin. Invest., 2010-1120(12):4236-50.
PMID: 21060150
There are no citations that match your criteria.
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