Recombinant Human Fc gamma RIIIB/CD16b Protein, CF

Catalog # 1597-FC/CF | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Accession #

O75015

Source

NS0

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Fc gamma RIIIB/CD16b protein
Thr20-Gln208, with a C-terminal 10-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr20

Predicted Molecular Mass

22.7 kDa

SDS-PAGE

40-60 kDa, reducing conditions

Activity

Measured by its ability to bind human IgG with an estimated KD

Scientific Data Images for Recombinant Human Fc gamma RIIIB/CD16b Protein, CF

Recombinant Human Fc gamma RIIIB/CD16b Protein Binding Activity

Recombinant Human Fc gamma RIIIB/CD16b Protein Binding Activity

Recombinant Human Fc gamma RIIIB/CD16b (Catalog # 1597-FC/CF) binds human IgG with an estimated Kd
Recombinant Human Fc gamma RIIIB/CD16b Protein SDS-PAGE

Recombinant Human Fc gamma RIIIB/CD16b Protein SDS-PAGE

1 μg/lane of Recombinant Human Fc gamma RIIIB/CD16b was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 43-60 kDa.

Formulation, Preparation and Storage

1597-FC/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc gamma RIIIB/CD16b

Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily. Based on their genetic organization and molecular structure, three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized (1 - 3). These receptors function in the activation or inhibition of immune responses. The activating-type receptor either has, or associates non-covalently with an accessory subunit (FcR gamma or zeta chain) that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. In contrast, the inhibitory receptor (Fc gamma RIIB) has a built-in immunoreceptor tyrosine-based inhibitory motif (ITIM) in its own cytoplasmic domain. Fc gamma RI is a high-affinity receptor that binds monomeric IgG. Both Fc gamma RII and RIII are low-affinity receptors that bind IgG in the form of immune complexes. Two genes for human Fc gamma RIII, A and B, encoding a transmembrane receptor and a glycosylphosphatidylinositol (GPI) anchored protein, respectively, have been identified. Three allelic variants of Fc gamma RIIIB, NA-1, NA-2, and SH, exist. A soluble form of Fc gamma RIIIB corresponding to the extracellular region of the receptor is produced by proteolytic cleavage and circulates in plasma and other body fluids. The extracellular domains of Fc gamma RIIIA and B share 97% amino acid sequence homology. Whereas Fc gamma RIIIA is expressed on most effector cells of the immune system including macrophage, monocyte, NK cells, mast cells, eosinophils, dendritic cells and Langerhans cells, Fc gamma RIIIB is selectively expressed in neutrophils and eosinophils. Signaling through Fc gamma RIIIA results in oxidative burst, cytokine release and phagocytosis by macrophages, antibody-dependent cellular cytotoxicity by natural killer cells and degranulation of mast cells. By contrast, Fc gamma RIIIB is a decoy receptor that binds IgG complexes without triggering activation. Soluble Fc gamma RIIIB has a regulatory role in inflammatory processes (4). It interacts with complement receptors CR3 and CR4 on monocytes to induce the production of pro-inflammatory cytokines.

References

  1. van de Winkel, J, and P. Capes (1993) Immunol. Today 14:215.
  2. Ravetch, J.V. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  3. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  4. Gauchat, G.J. et al. (1996) J. Immunol. 157:1184.

Long Name

Fc gamma Receptor III

Alternate Names

CD16b, FCG3, FCGR3B, FcgRIIIB, FcR-10, FCRIIIB, IGFR3

Entrez Gene IDs

2215 (Human); 14131 (Mouse)

Gene Symbol

FCGR3B

UniProt

O75015

Product Documents for Recombinant Human Fc gamma RIIIB/CD16b Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Fc gamma RIIIB/CD16b Protein, CF

For research use only

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