Recombinant Human CXCL7/NAP-2 Protein

Name: Recombinant Human CXCL7/NAP-2 Protein
Brand: R&D Systems
SKU: 393-NP

Catalog # 393-NP | R&D Systems, Inc. a Bio-Techne Brand

Citations (7)

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Key Product Details

Source:	E. coli
Accession #:	P02775
Applications:	Bioactivity

View Full Technical Details for Recombinant Human CXCL7/NAP-2 Protein

Technical Data
Citations (7) / Reviews

Product Specifications for Recombinant Human CXCL7/NAP-2 Protein

Source	E. coli-derived human CXCL7/NAP-2 protein Ala59-Asp128
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala59
Predicted Molecular Mass	7.6 kDa
SDS-PAGE	7.6 kDa
Activity	Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED₅₀ for this effect is 0.200-1.60 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

393-NP

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

393-NP/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCL7/NAP-2

Neutrophil Activating Peptide 2 (NAP-2), Connective Tissue Activating Protein III (CTAP-III) and beta -thrombogulin ( beta -TG), are proteolytically processed carboxyl-terminal fragments of platelet basic protein (PBP) which is found in the alpha-granules of human platelets. NAP-2 is a member of the CXC chemokines. Similar to other ELR domain containing CXC chemokines such as IL-8 and the GRO proteins, NAP-2 has been shown to bind CXCR2 and to chemoattract and activate neutrophils. Although CTAP-III, beta -TG and PBP represent amino-terminal extended variants of NAP-2 and possess the same CXC chemokine domains, these proteins do not exhibit NAP-2 activity. It has been shown that the additional amino-terminal residues of CTAP-III masks the critical ELR receptor binding domain that is exposed on NAP-2 and may account for lack of NAP-2 activity.

Item	Value
References	Schall, T. (1994) The Cytokine Handbook, 2nd edition, A. Thomson, ed. Academic Press, New York, p. 419. Malkowski, M.G. et al. (1997) J. Mol. Biol. 266:367.
Entrez Gene IDs	5473 (Human)
Alternate Names	Beta-TG, C-X-C motif chemokine 7, CTAP III, CTAP3CTAP-III, CTAPIII, CXC chemokine ligand 7, CXCL7, CXCL7b-TG1, LA-PF4, LDGFTC1, Leukocyte-derived growth factor, MDGFTC2, Macrophage-derived growth factor, NAP-2, NAP-2-L1, NAP2, PBPTHBGB, PPBP, SCAR10, SCYB7beta-thromboglobulin, Small-inducible cytokine B7, TGB, TGB1B-TG1, THBGB1, connective tissue-activating peptide III, low-affinity platelet factor IV, neutrophil-activating peptide 2, neutrophil-activating peptide-2, platelet basic protein, pro-platelet basic protein (chemokine (C-X-C motif) ligand 7), small inducible cytokine B7, small inducible cytokine subfamily B, member 7, thrombocidin 1, thrombocidin 2, thromboglobulin, beta-1

Citations for Recombinant Human CXCL7/NAP-2 Protein (7)

Showing 1 - 7 of 7 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human CXCL7/NAP-2 Protein include:

Species: Human
Sample Types: Plasma
Applications: Bioassay

K Egan et al. (2017-03-30), Effect of platelet-derived ?-thromboglobulins on coagulation Thromb. Res., 2017-0154(0):7-15.
PMID: 28384443
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2012-07-31), Staphylococcus aureus Staphopain A inhibits CXCR2-dependent neutrophil activation and chemotaxis. EMBO J., 2012-031(17):3607-19.
PMID: 22850671
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wilson TR et al. (2012-07-26), Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-0487(7408):505-9.
PMID: 22763448
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kirouac DC et al. (2010-10-05), Dynamic interaction networks in a hierarchically organized tissue. Mol. Syst. Biol., 2010-16(0):417.
PMID: 20924352
Species: Mouse
Sample Types: Complex Sample Type
Applications: Binding Assay

Fan X et al. (2006-12-29), Murine CXCR1 is a functional receptor for GCP-2/CXCL6 and interleukin-8/CXCL8. J. Biol. Chem., 2006-1282(16):11658-66.
PMID: 17197447
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Smith C et al. (2006-09-27), Increased levels of neutrophil-activating peptide-2 in acute coronary syndromes: possible role of platelet-mediated vascular inflammation. J. Am. Coll. Cardiol., 2006-048(8):1591-9.
PMID: 17045893
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Traves SL et al. (2004-05-20), Specific CXC but not CC chemokines cause elevated monocyte migration in COPD: a role for CXCR2. J. Leukoc. Biol., 2004-076(2):441-50.
PMID: 15155777

Recombinant Human CXCL7/NAP-2 Protein

Catalog # 393-NP | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Recombinant Human CXCL7/NAP-2 Protein

Formulation, Preparation and Storage

Carrier Free

393-NP

Carrier

393-NP/CF

Carrier Free

Reconstitution Calculator

Background: CXCL7/NAP-2

Citations for Recombinant Human CXCL7/NAP-2 Protein (7)

Product Documents for Recombinant Human CXCL7/NAP-2 Protein

Product Datasheets

COA

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SDS

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