Recombinant Human CHST15 Protein, CF

Name: Recombinant Human CHST15 Protein, CF
Brand: R&D Systems
SKU: 3365-ST

Catalog # 3365-ST | R&D Systems, Inc. a Bio-Techne Brand

Citations (1)

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Key Product Details

Source:	NS0
Accession #:	Q7LFX5
Applications:	Enzyme Activity

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Technical Data
Citations (1) / Reviews

Product Specifications for Recombinant Human CHST15 Protein, CF

Source	Mouse myeloma cell line, NS0-derived human Carbohydrate Sulfotransferase 15/CHST15 protein Ser99-Thr561, with an N-terminal 6-His tag
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	His
Predicted Molecular Mass	55 kDa
SDS-PAGE	55 kDa
Activity	Measured by its ability to transfer sulfate from PAPS to chondroitin sulfate. The specific activity is >100 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3365-ST

Formulation	Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

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Background: Carbohydrate Sulfotransferase 15/CHST15

N‑Acetylgalactosamine 4‑sulfate 6‑O‑sulfotransferase (GalNAc4S‑6ST, or, CHST15) is a type II transmembrane protein composed of a short N‑terminal cytoplasmic domain and a long C‑terminal luminal catalytic domain. GalNAc4S‑6ST transfers sulfate from 3'‑phosphoadenosine 5'‑phosphosulfate (PAPS) to the 6‑O of GalNAc‑4S on chondroitin sulfate A (CS‑A), as well as dermatan sulfate (1), generating the chondroitin sulfate E (CS‑E) disaccharide unit GlcA beta 1‑3GalNAc‑4S,6S. CS‑E binds with strong affinity to Midkine, a heparan sulfate‑binding growth factor (2), playing an important regulatory role in differentiation and morphogenesis during embryonic development. In situ hybridization for the expression of GalNAc4S‑6ST in the postnatal mouse brain showed a widespread expression of the transcript in the developing brain except at postnatal day 7 (3). For comparison, chondroitin sulfate 6‑O sulfotransferase‑1 (C6ST‑1) encoded by the CHST3 gene, only recognizes and transfers sulfate to the 6‑O of unsulfated GalNAc residues on chondroitin sulfate generating the chondroitin sulfate C (CS‑C) disaccharide unit GlcA beta 1‑3GalNAc‑6S (4). The sequence of the human GalNAc4S‑6ST was found to be the same as human B‑cell RAG‑associated protein (BRAG) (1). The activity of the recombinant human CHST15 is measured using a PAP-specific phosphatasecoupled sulfotransferase assay (5).

Item	Value
References	Ohtake, S. et al. (2001) J. Biol. Chem. 276:43894. Zou, P. et al. (2003) Glycobiology 13:35. Purushothaman, A. et al. (2007) J. Biol. Chem. 282:19442. Thiele, H. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10155. Prather, B. et al. (2012) Anal. Biochem. 423:86.
Entrez Gene IDs	51363 (Human); 286974 (Rat)
Alternate Names	B cell RAG associated protein (GALNAC4S-6ST), B-cell RAG-associated gene protein, BRAG, BRAGKIAA0598RP11-47G11.1, CHST15, Carbohydrate Sulfotransferase 15, DKFZp781H1369, EC 2.8.2.33, GALNAC4S6ST, GalNAc4S-6ST, MGC34346, N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase, carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15, hBRAG

Citations for Recombinant Human CHST15 Protein, CF (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human CHST15 Protein, CF include:

Species: Bovine, Porcine
Sample Types: Heparin, Protein
Applications: Enzyme Assay

Wu ZL et al. (2010-12-17), Detection of specific glycosaminoglycans and glycan epitopes by in vitro sulfation using recombinant sulfotransferases. Glycobiology, 2010-121(5):625-33.
PMID: 21169395

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Product Documents for Recombinant Human CHST15 Protein, CF

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