Recombinant Human NSDHL His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23258

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NBP2-23258
NBP2-23258-0.05mg
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all NSDHL Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-297 of Human NSDHL

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMEPAVSE PMRDQVARTH LTEDTPKVNA DIEKVNQNQA KRCTVIGGSG FLGQHMVEQL LARGYAVNVF DIQQGFDNPQ VRFFLGDLCS RQDLYPALKG VNTVFHCASP PPSSNNKELF YRVNYIGTKN VIETCKEAGV QKLILTSSAS VIFEGVDIKN GTEDLPYAMK PIDYYTETKI LQERAVLGAN DPEKNFLTTA IRPHGIFGPR DPQLVPILIE AARNGKMKFV IGNGKNLVDF TFVENVVHGH ILAAEQLSRD STLGGKAFHI TNDEPIPFWT FLSRILTGLN YEAPKYHIPY

Purity

>80%, by SDS-PAGE

Predicted Molecular Mass

35.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human NSDHL His Protein

SDS-PAGE: Recombinant Human NSDHL His Protein [NBP2-23258]

SDS-PAGE: Recombinant Human NSDHL His Protein [NBP2-23258]

SDS-Page: NSDHL Protein [NBP2-23258]

Formulation, Preparation and Storage

NBP2-23258
Formulation 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.1 M NaCl
Preservative No Preservative
Concentration 0.25 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NSDHL

NSDHL, as known as Sterol-4-alpha-carboxylate 3-dehydrogenase, is involved in the production (synthesis) of cholesterol. During cholesterol synthesis, it participates in one of several steps that convert a molecule called lanosterol to cholesterol. Specifically, this enzyme removes a carbon atom and three hydrogen atoms (a methyl group) in the conversion of lanosterol to cholesterol. It is also an important component of cell membranes and myelin, the fatty covering that insulates nerve cells. Recombinant human NSDHL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

EC 1.1.1.170, H105E3, member 1, NAD(P) dependent steroid dehydrogenase-like, SDR31E1, sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating, XAP104

Gene Symbol

NSDHL

Additional NSDHL Products

Product Documents for Recombinant Human NSDHL His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human NSDHL His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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