Mouse IL-36 alpha/IL-1F6 DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY2297-05
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
78.1-5000 pg/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Mouse
Mouse IL-36 alpha/IL-1F6 DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Mouse IL-36 alpha/IL-1F6 DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse IL-36. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
100 µL
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Mouse IL-36 alpha/IL-1F6 DuoSet ELISA
Mouse IL-36 alpha/IL-1F6 DuoSet ELISA Standard Curve
Kit Contents for Mouse IL-36 alpha/IL-1F6 DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008C) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: ELISA TMB Substrate (Catalog # DY999B or DY999B-250)
Stop Solution: Methanesulfonic acid (Catalog # DY994B or DY994B-250)
Microplates: (Catalog # DY990), or equivalent
Plate Sealers: (Catalog # DY992), or equivalent
*For the recommended Reagent Diluent and Blocking Buffer for a specific DuoSet ELISA Development Kit, refer to the product datasheet.
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-36 alpha/IL-1F6
Interleukin 36 alpha (IL-36 alpha), also known as IL-1F6 and FIL1 epsilon, is a member of the IL-1 family of proteins. Cells reported to express IL-36 alpha include monocytes, B cells, and T cells. Notably, IL-36 alpha is the only novel IL-1 family member expressed on T-cells. The receptor for IL-36 alpha is reported to be a combination of IL-1 R6/IL-1 R rp2 and IL-1 R3/IL-1 R AcP. Recombinant IL-36 alpha has been shown to activate pathways involving NF-kappa B and MAPK in an IL-1 R6/IL-1 R rp2-dependent manner.
Long Name
Interleukin 36 alpha/Interleukin 1 Family 6
Alternate Names
FIL1 epsilon, IL-1E, IL1F6, IL36 alpha, IL36A
Gene Symbol
IL36A
Additional IL-36 alpha/IL-1F6 Products
Product Documents for Mouse IL-36 alpha/IL-1F6 DuoSet ELISA
Product Specific Notices for Mouse IL-36 alpha/IL-1F6 DuoSet ELISA
For research use only
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