Human Aminopeptidase N/CD13 DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY3815
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
0.312-20 ng/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human
Human Aminopeptidase N/CD13 DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human Aminopeptidase N/CD13 DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Aminopeptidase N / CD13. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
100 µL
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human Aminopeptidase N/CD13 DuoSet ELISA
Human Aminopeptidase N / CD13 ELISA Standard Curve
Kit Contents for Human Aminopeptidase N/CD13 DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008C) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: ELISA TMB Substrate (Catalog # DY999B or DY999B-250)
Stop Solution: Methanesulfonic acid (Catalog # DY994B or DY994B-250)
Microplates: (Catalog # DY990), or equivalent
Plate Sealers: (Catalog # DY992), or equivalent
*For the recommended Reagent Diluent and Blocking Buffer for a specific DuoSet ELISA Development Kit, refer to the product datasheet.
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Aminopeptidase N/CD13
The ANPEP gene encodes Aminopeptidase N (APN), also known as CD13 or Alanyl Aminopeptidase. The deduced amino acid sequence of APN consists of a short cytoplasmic tail, a transmembrane region, a Ser/Thr rich region, and a zinc metalloproteinase domain. Widely expressed in many cells, tissues, and species, APN cleaves the N-terminal amino acids from bioactive peptides, leading to their inactivation or degradation. It has putative involvement in several biological processes including antigen processing and presentation, cell adhesion, tumor invasion and metastasis, neurotransmitter degradation, and it also serves as a receptor for certain coronaviruses.
Alternate Names
Aminopeptidase M, ANPEP, APN, CD13, gp150, PEPN
Gene Symbol
ANPEP
Additional Aminopeptidase N/CD13 Products
Product Documents for Human Aminopeptidase N/CD13 DuoSet ELISA
Product Specific Notices for Human Aminopeptidase N/CD13 DuoSet ELISA
For research use only
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