25-hydroxy Vitamin D3 ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66361
Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma and other biological fluids (50 uL)
Sensitivity
1.88 ng/mL
Assay Range
3.13 - 200 ng/mL
Product Specifications
Assay Type
Competitive-ELISA
Kit Type
ELISA Kit (Colorimetric)
Specificity
This kit recognizes 25-hydroxy Vitamin D3 in samples. No significant cross-reactivity or interference between 25-hydroxy Vitamin D3 and analogues was observed.
Precision
Intra-Assay Precision (Precision within an assay) CV% < 5.02%
Inter-Assay Precision (Precision between assays) CV% < 4.96%
Recovery for 25-hydroxy Vitamin D3 ELISA Kit (Colorimetric)
Recovery
87-112%
Linearity
Scientific Data Images for 25-hydroxy Vitamin D3 ELISA Kit (Colorimetric)
Universal 25-hydroxy Vitamin D3 ELISA Kit (Colorimetric) [NBP2-66361] - Standard Curve Reference
ELISA: 25-hydroxy Vitamin D3 ELISA Kit (Colorimetric) [NBP2-66361] -
ELISA: 25-hydroxy Vitamin D3 ELISA Kit (Colorimetric) [NBP2-66361] - Standard Curve ReferenceKit Contents for 25-hydroxy Vitamin D3 ELISA Kit (Colorimetric)
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: 25-hydroxy Vitamin D3
Alternate Names
25-Hydroxycholecalciferol, 25-Hydroxyvitamin D, 25-hydroxyvitamin D3, 25-OH D3, 25-OH Vitamin D3
Additional 25-hydroxy Vitamin D3 Products
Product Documents for 25-hydroxy Vitamin D3 ELISA Kit (Colorimetric)
Product Specific Notices for 25-hydroxy Vitamin D3 ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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