Western blot shows recombinant Japanese Encephalitis virus NS1 (rvJEV NS1). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Viral Japanese Encephalitis virus NS1 Monoclonal Antibody (Catalog # MAB10006) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Japanese Encephalitis virus NS1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Viral Japanese Encephalitis virus NS1 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Japanese Encephalitis virus NS1

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Japanese Encephalitis virus NS1

Japanese encephalitis virus (JEV) non-structural protein 1 (NS1) is produced by the Japanese encephalitis virus (JEV), a mosquito-borne flavivirus that is the leading cause of virus-induced encephalitis globally (1). Flavivirus NS1 is a multifunctional virulence factor. The glycosylated NS1 exists as a membrane-associated dimer after translocation into the endoplasmic reticulum lumen, where it is essential for viral genome replication (2). The secreted hexamer NS1 is involved in immune evasion and pathogenesis; it is identified as a potential diagnostic marker for early detection of the virus infections (2). Mature viral JEV NS1 contains 352 amino acids and shares high structural similarity to other flavivirus NS1 proteins, such as DENV and ZIKV.

References

Nain, M. et al. (2016) Rev. Med. Virol. 26:129.
Rastogi, M. et al. (2016) Virol. J. 13:131.

Alternate Names

JE virus, JEV, JEV-NS1, JEVgp1

Entrez Gene IDs

1489713 (Viral)

Gene Symbol

POLY

UniProt

P32886

Additional Japanese Encephalitis virus NS1 Products

Product Documents for Viral Japanese Encephalitis virus NS1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Viral Japanese Encephalitis virus NS1 Antibody

For research use only

Citations
Reviews

Viral Japanese Encephalitis virus NS1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10006

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Japanese Encephalitis virus NS1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Viral Japanese Encephalitis virus NS1 Antibody

Detection of Japanese Encephalitis virus NS1 by Western Blot.